1XL3	Cell Invasion	date	Sep 30, 2004
title	Complex Structure Of Y.Pestis Virulence Factors Yopn And Tyea
authors	F.D.Schubot, M.W.Jackson, K.J.Penrose, S.Cherry, J.E.Tropea, G.V.Plano, D.S.Waugh
compound		source
Molecule: Secretion Control Protein Chain: A, B Synonym: Yop4b, Lcre Engineered: Yes Mutation: Yes		Organism_scientific: Yersinia Pestis Organism_common: Bacteria Gene: Yopn, Lcre Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pdest42
Molecule: Protein Type A Chain: C, D Engineered: Yes		Organism_scientific: Yersinia Pestis Organism_common: Bacteria Gene: Tyea Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pdest42
symmetry	Space Group: P 21 21 2	R_factor	0.192
crystal cell	length a length b length c angle alpha angle beta angle gamma 82.172 170.724 55.580 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.20 Å
ligand	MLY	enzyme
note	1XL3 is a representative structure
similarity	Belongs to the HrpJ/yopn family.
subunit	Interacts with the translocator yopd and with residues 242-293 of yopn (by similarity).
subcellular loc.	Outer membrane (by similarity).
Genes	TYEA, Y0043, Y5040, YPCD1.38C (Y. pestis); YP (Y. pestis)
function	Seems to sense the calcium concentration and to transmit a signal to shut off yop transcription when the calcium concentration is high (by similarity). Plays a major role in regulation of the low-calcium response. Involved in the control of yop release (by similarity).
Gene Ontology	Chain Function Process Component A, B calcium ion binding pathogenesis protein secretion by the typ... secretion negative regulation of prote... cell surface membrane outer membrane
Primary reference	Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis., Schubot FD, Jackson MW, Penrose KJ, Cherry S, Tropea JE, Plano GV, Waugh DS, J Mol Biol 2005 Mar 4;346(4):1147-61. Epub 2005 Jan 20. PMID:15701523

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (106 Kb) [Save to disk] Biological Unit Coordinates (1xl3.pdb1.gz) 53 Kb Biological Unit Coordinates (1xl3.pdb2.gz) 49 Kb CSU: Contacts of Structural Units for 1XL3 Likely Quarternary Molecular Structure file(s) for 1XL3 Structure Factors (309 Kb) Retrieve 1XL3 in mmCIF format [Save to disk]

View 1XL3 in 3D

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1XL3, from MSDmotif at EBI Fold representative 1xl3 from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1xl3_A] [1xl3_D] [1xl3_B] [1xl3_C]

Other resources with information on 1XL3

InterPro: IPR010812 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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