1VA5	Transferase	date	Feb 11, 2004
title	Antigen 85c With Octylthioglucoside In Active Site
authors	D.R.Ronning, V.Vissa, G.S.Besra, J.T.Belisle, J.C.Sacchettini, Tb Structural Genomics Consortium (Tbsgc)
compound		source
Molecule: Antigen 85-C Chain: A, B Ec: 2.3.1.- Engineered: Yes		Organism_scientific: Mycobacterium Tuberculosis Organism_common: Bacteria Gene: Fbpc2 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 (De3) Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet29
symmetry	Space Group: C 1 2 1	R_factor	0.187
crystal cell	length a length b length c angle alpha angle beta angle gamma 144.818 68.117 82.281 90.00 119.60 90.00
method	X-Ray Diffraction	resolution	2.02 Å
ligand	SOG	enzyme	Transferase E.C.2.3.1 BRENDA
similarity	Belongs to the mycobacterial a85 antigen family.[Esterase]
subcellular loc.	Secreted.
function	Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.
Gene Ontology	Chain Function Process Component A, B acyltransferase activity transferase activity
Primary reference	Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity., Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC, J Biol Chem 2004 Aug 27;279(35):36771-7. Epub 2004 Jun 10. PMID:15192106

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (112 Kb) [Save to disk] Biological Unit Coordinates (1va5.pdb1.gz) 52 Kb Biological Unit Coordinates (1va5.pdb2.gz) 46 Kb CSU: Contacts of Structural Units for 1VA5 Likely Quarternary Molecular Structure file(s) for 1VA5 Structure Factors (374 Kb) Retrieve 1VA5 in mmCIF format [Save to disk]

View 1VA5 in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1VA5

Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only) Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges. STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure. GRASS (Graphical Representation and Analysis of Structure Server) Cartoon representation from PDB Cartoon Ramachandran plot from PDBSum

Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1VA5, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1va5a_, region A [Jmol] [rasmolscript] [script source]     - Domain d1va5b_, region B [Jmol] [rasmolscript] [script source] Fold representative 1va5 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1va5_A] [1va5_B]

Other resources with information on 1VA5

InterPro: IPR000801 MMDB (Entrez's Structure Database)

Movements, Movies and Images

Images from IMB Jena Image Library of Biological Macromolecules.

You may enter another PDB ID code

Go [Back], to the [PDB Lite page], to the [OCA Search page] or to the [PDB Home page]

---

OCA© by Jaime Prilusky, 1996-2006
Bioinformatics and Biological Computing
Weizmann Institute of Science

---