1T13	Transferase	date	Apr 15, 2004
title	Crystal Structure Of Lumazine Synthase From Brucella Abortus Bound To 5-Nitro-6-(D-Ribitylamino)-2,4(1h,3h) Pyrimidinedione
authors	S.Klinke, V.Zylberman, D.R.Vega, B.G.Guimaraes, B.C.Braden, F.A.Goldbaum
compound		source
Molecule: 6,7-Dimethyl-8-Ribityllumazine Synthase Chain: A, B, C, D, E Synonym: Dmrl Synthase, Lumazine Synthase, Riboflavin Synthase Beta Chain; Ec: 2.5.1.9 Engineered: Yes		Organism_scientific: Brucella Melitensis Biovar Abortus Organism_common: Bacteria Gene: Ribh, Bmeii0589, Bra0695 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21(De3) Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet11b
symmetry	Space Group: P 31 2 1	R_factor	0.217
crystal cell	length a length b length c angle alpha angle beta angle gamma 126.321 126.321 165.669 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.90 Å
ligand	INI, PO4	enzyme	Transferase E.C.2.5.1.9 BRENDA
related structures	by homologous chain: 1DI0
Gene Ontology	Chain Function Process Component A, D, C, E, B riboflavin synthase activity... transferase activity riboflavin biosynthetic proc... riboflavin synthase complex
Primary reference	Crystallographic Studies on Decameric Brucella spp. Lumazine Synthase: A Novel Quaternary Arrangement Evolved for a New Function?, Klinke S, Zylberman V, Vega DR, Guimaraes BG, Braden BC, Goldbaum FA, J Mol Biol 2005 Oct 14;353(1):124-37. PMID:16165152

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (124 Kb) [Save to disk] Biological Unit Coordinates (1t13.pdb1.gz) 238 Kb CSU: Contacts of Structural Units for 1T13 Likely Quarternary Molecular Structure file(s) for 1T13 Structure Factors (526 Kb) Retrieve 1T13 in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1T13, from MSDmotif at EBI Fold representative 1t13 from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1t13_D] [1t13_B] [1t13_A] [1t13_E] [1t13_C]

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