1SFR	Transferase	date	Feb 20, 2004
title	Crystal Structure Of The Mycobacterium Tuberculosis Antigen 85a Protein
authors	D.R.Ronning, V.Vissa, G.S.Besra, J.T.Belisle, J.C.Sacchettini, Tb Structural Genomics Consortium (Tbsgc)
compound		source
Molecule: Antigen 85-A Chain: A, B, C Ec: 2.3.1.- Engineered: Yes		Organism_scientific: Mycobacterium Tuberculosis Organism_common: Bacteria Gene: Fbpa Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21(De3) Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet30b
symmetry	Space Group: C 2 2 21	R_factor	0.222
crystal cell	length a length b length c angle alpha angle beta angle gamma 131.430 288.662 101.289 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.70 Å
ligand		enzyme	Transferase E.C.2.3.1 BRENDA
similarity	Belongs to the mycobacterial a85 antigen family.[Esterase]
post-translat. modifications	Contains one disulfide bridge.
subcellular loc.	Membrane localization by OPM: Bacterial gram-positive acid-fast cell wall out side in side
function	Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.
Gene Ontology	Chain Function Process Component A, C, B acyltransferase activity transferase activity
Primary reference	Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity., Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC, J Biol Chem 2004 Aug 27;279(35):36771-7. Epub 2004 Jun 10. PMID:15192106

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (152 Kb) [Save to disk] Biological Unit Coordinates (1sfr.pdb1.gz) 47 Kb Biological Unit Coordinates (1sfr.pdb2.gz) 46 Kb Biological Unit Coordinates (1sfr.pdb3.gz) 44 Kb CSU: Contacts of Structural Units for 1SFR Likely Quarternary Molecular Structure file(s) for 1SFR Structure Factors (437 Kb) Retrieve 1SFR in mmCIF format [Save to disk]

View 1SFR in 3D

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Visual 3D analysis of 1SFR

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1SFR, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1sfra_, region A [Jmol] [rasmolscript] [script source]     - Domain d1sfrb_, region B [Jmol] [rasmolscript] [script source]     - Domain d1sfrc_, region C [Jmol] [rasmolscript] [script source] Fold representative 1sfr from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1sfr_A] [1sfr_B] [1sfr_C]

Other resources with information on 1SFR

InterPro: IPR000801 MMDB (Entrez's Structure Database)

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