1RCV | Toxin | date | Nov 04, 2003 ![]() |
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title | Cholera Toxin B-Pentamer Complexed With Bivalent Nitrophenol-Galactoside Ligand Bv1 | ||||||||||||||
authors | J.C.Pickens, D.D.Mitchell, J.Liu, X.Tan, Z.Zhang, C.L.Verlinde, W.G.Hol, E.Fan | ||||||||||||||
compound | source | ||||||||||||||
Molecule: Cholera Toxin B Protein (Ctb) Chain: D, E, F, G, H Engineered: Yes |
Organism_scientific: Vibrio Cholerae Organism_common: Bacteria Gene: Ctxb Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Top10 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pbad | ||||||||||||||
symmetry | Space Group: C 1 2 1 | R_factor | 0.164 | ||||||||||||
crystal cell |
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method | X-Ray Diffraction | resolution | 1.60 Å | ||||||||||||
ligand | BV1 | enzyme |
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similarity | Belongs to the Enterotoxin_b family. | ||||||||||||||
subunit | The a subunit contains two chains, a1 and a2, linked by a disulfide bridge. The holotoxin (choleragen) consists of a pentameric ring of b subunits whose central pore is occupied by the a subunit. | ||||||||||||||
gene | ctxB (V. cholerae) | ||||||||||||||
function | It can bind five gm1 gangliosides. The b subunit pentameric ring directs the a subunit to its target by binding to the gm1 gangliosides present on the surface of the intestinal epithelial cells. It has no toxic activity by itself. | ||||||||||||||
Gene Ontology |
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Primary reference | Nonspanning bivalent ligands as improved surface receptor binding inhibitors of the cholera toxin B pentamer., Pickens JC, Mitchell DD, Liu J, Tan X, Zhang Z, Verlinde CL, Hol WG, Fan E, Chem Biol 2004 Sep;11(9):1205-15. PMID:15380181 |
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Structure-derived information |
- Domain d1rcvd_, region D [Jmol] [rasmolscript] [script source] - Domain d1rcve_, region E [Jmol] [rasmolscript] [script source] - Domain d1rcvf_, region F [Jmol] [rasmolscript] [script source] - Domain d1rcvg_, region G [Jmol] [rasmolscript] [script source] - Domain d1rcvh_, region H [Jmol] [rasmolscript] [script source] |
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