1PSZ	Immune System	date	Oct 13, 1998
title	Pneumococcal Surface Antigen Psaa
authors	M.C.Lawrence, P.A.Pilling, V.C.Epa, A.M.Berry, A.D.Ogunniyi, J.C.Paton
compound		source
Molecule: Surface Antigen Psaa Chain: A Engineered: Yes Mutation: Yes Other_details: The Expressed Construct Lacks The Pro- Lipoprotein Recognition Sequence Lxxc		Organism_scientific: Streptococcus Pneumoniae Organism_common: Pneumococcus Strain: D39 (Serotype 2) Collection: Nctc7466 Cellular_location: Extracellular Expression_system: Escherichia Coli Expression_system_strain: Sg12009 [Prep4] Expression_system_cellular_location: Cytoplasm Expression_system_vector_type: Qiaexpress Expression_system_vector: Pqe30
symmetry	Space Group: P 21 21 21	R_factor	0.178
crystal cell	length a length b length c angle alpha angle beta angle gamma 60.024 66.517 69.838 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.00 Å
ligand	ZN	enzyme
note	1PSZ is a representative structure
similarity	Belongs to the bacterial solute-binding protein 9 family. Lipoprotein receptor antigen (lrai) subfamily.[SBP_bac_9]
subcellular loc.	Attached to the membrane by a lipid anchor (by similarity).
gene	psaA (S. pneumoniae)
function	Part of an atp-driven transport system for manganese. It is an important factor in pathogenesis and infection. Also act as an adhesin which is involved on adherence to extracellular matrix.
Gene Ontology	Chain Function Process Component A binding manganese ion binding transport cell adhesion membrane outer membrane-bounded perip...
Primary reference	The crystal structure of pneumococcal surface antigen PsaA reveals a metal-binding site and a novel structure for a putative ABC-type binding protein., Lawrence MC, Pilling PA, Epa VC, Berry AM, Ogunniyi AD, Paton JC, Structure 1998 Dec 15;6(12):1553-61. PMID:9862808

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (62 Kb) [Save to disk] Biological Unit Coordinates (1psz.pdb1.gz) 51 Kb CSU: Contacts of Structural Units for 1PSZ Likely Quarternary Molecular Structure file(s) for 1PSZ Structure Factors (147 Kb) Retrieve 1PSZ in mmCIF format [Save to disk]

View 1PSZ in 3D

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Visual 3D analysis of 1PSZ

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1PSZ, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1psza_, region A [Jmol] [rasmolscript] [script source] Fold representative 1psz from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1psz_A]

Other resources with information on 1PSZ

InterPro: IPR006128 , IPR006127 , IPR006129 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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