1P4T Membrane Protein date Apr 24, 2003
title Crystal Structure Of Neisserial Surface Protein A (Nspa)
authors L.Vandeputte-Rutten, M.P.Bos, J.Tommassen, P.Gros
compound source
Molecule: Outer Membrane Protein Nspa
Chain: A
Synonym: Neisserial Surface Protein A, Nspa
Engineered: Yes
 Organism_scientific: Neisseria Meningitidis
Gene: Nspa
Expression_system: Escherichia Coli
Expression_system_common: Bacteria
Expression_system_strain: Bl21(De3)
Expression_system_vector_type: Plasmid
Expression_system_plasmid: Pet11a
symmetry Space Group: H 3 2
R_factor 0.225
crystal
cell
length a length b length c angle alpha angle beta angle gamma
97.372 97.372 171.939 90.00 90.00 120.00
method X-Ray Diffractionresolution 2.55 Å
ligand CXE, ETA, SO4 enzyme
note 1P4T is a representative structure
subcellular loc. Membrane localization by OPM: Bacterial gram-negative outer membrane
extracellular side
periplasmic side
Gene
Ontology
ChainFunctionProcessComponent
A
  • porin activity

  • membrane
    • Primary referenceCrystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential., Vandeputte-Rutten L, Bos MP, Tommassen J, Gros P, J Biol Chem 2003 Jul 4;278(27):24825-30. PMID:12716881
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (38 Kb) [Save to disk]
  • Biological Unit Coordinates (1p4t.pdb1.gz) 26 Kb
  • CSU: Contacts of Structural Units for 1P4T
  • Likely Quarternary Molecular Structure file(s) for 1P4T
  • Structure Factors (88 Kb)
  • Retrieve 1P4T in mmCIF format [Save to disk]
    • View 1P4T in 3D
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  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1p4ta_, region A [Jmol] [rasmolscript] [script source]
  • Fold representative 1p4t from FSSP and Dali (Families of Structurally Similar Proteins)
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  • View one-letter amino acid or nucleotide sequence for each chain: [1p4t_A]
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