1P4P	Membrane Protein	date	Apr 23, 2003
title	Outer Surface Protein B Of B. Burgdorferi: Crystal Structure Of The C-Terminal Fragment
authors	M.Becker, J.Bunikis, B.D.Lade, J.J.Dunn, A.G.Barbour, C.L.Lawson
compound		source
Molecule: Outer Surface Protein B Chain: A Fragment: Ospb C-Terminal Fragment Synonym: Ospb Engineered: Yes		Organism_scientific: Borrelia Burgdorferi Organism_common: Lyme Disease Spirochete Gene: Ospb Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl2(De3)Plyss Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet9c
symmetry	Space Group: C 2 2 21	R_factor	0.211
crystal cell	length a length b length c angle alpha angle beta angle gamma 30.800 51.660 158.820 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.00 Å
note	1P4P is a representative structure
related structures	by homologous chain: 1RJL
similarity	Belongs to the Lipoprotein_1 family.
subcellular loc.	Attached to the outer membrane by a lipid anchor.
Gene	BB ; OSPB (B. burgdorferi)
Gene Ontology	Chain Function Process Component A cell outer membrane membrane outer membrane
Primary reference	Structural Investigation of Borrelia burgdorferi OspB, a BactericidalFab Target., Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL, J Biol Chem 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:15713683

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (28 Kb) [Save to disk] Biological Unit Coordinates (1p4p.pdb1.gz) 25 Kb CSU: Contacts of Structural Units for 1P4P Likely Quarternary Molecular Structure file(s) for 1P4P Retrieve 1P4P in mmCIF format [Save to disk]

View 1P4P in 3D

Proteopedia, because life has more than 2D. On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1P4P

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1P4P, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1p4pa_, region A [Jmol] [rasmolscript] [script source] Fold representative 1p4p from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1p4p_A]

Other resources with information on 1P4P

InterPro: IPR001809 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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