1P3H	Chaperone	date	Apr 17, 2003
title	Crystal Structure Of The Mycobacterium Tuberculosis Chaperonin 10 Tetradecamer
authors	M.M.Roberts, A.R.Coker, G.Fossati, P.Mascagni, A.R.M.Coates, S.P.Wood, Tb Structural Genomics Consortium (Tbsgc)
compound		source
Molecule: 10 Kda Chaperonin Chain: A, B, C, D, E, F, G, H, I, J, K, L, M, N Synonym: Protein Cpn10, Groes Protein, Bcg-A Heat Shock Protein, 10 Kda Antigen; Engineered: Yes		Organism_scientific: Mycobacterium Tuberculosis Organism_common: Bacteria Gene: Gros Or Groes Or Mopb Or Cpn10 Or Rv3418c Or Mt3527 Or Mtcy78.11; Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 (De3) Expression_system_vector_type: Plasmid Expression_system_plasmid: Puc18
symmetry	Space Group: P 1 21 1	R_factor	0.259
crystal cell	length a length b length c angle alpha angle beta angle gamma 76.460 87.930 124.390 90.00 106.78 90.00
method	X-Ray Diffraction	resolution	2.80 Å
ligand	CA, MPD	enzyme
note	1P3H is a representative structure and supersedes 1JH2
similarity	Belongs to the groes chaperonin family.[Cpn10]
subunit	Heptamer of 7 subunits arranged in a ring (by similarity).
subcellular loc.	Cytoplasm (by similarity).
gene	MT3527 (M. tuberculosis)
function	Binds to cpn60 in the presence of mg-atp and suppresses the atpase activity of the latter.
Gene Ontology	Chain Function Process Component F, A, J, N, K, E, B, H, M, C, D, I, G, L ATP binding unfolded protein binding protein folding response to unfolded protein...
Primary reference	Mycobacterium tuberculosis chaperonin 10 heptamers self-associate through their biologically active loops., Roberts MM, Coker AR, Fossati G, Mascagni P, Coates AR, Wood SP, J Bacteriol 2003 Jul;185(14):4172-85. PMID:12837792

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (248 Kb) [Save to disk] Biological Unit Coordinates (1p3h.pdb1.gz) 112 Kb Biological Unit Coordinates (1p3h.pdb2.gz) 111 Kb CSU: Contacts of Structural Units for 1P3H Likely Quarternary Molecular Structure file(s) for 1P3H Structure Factors (281 Kb) Retrieve 1P3H in mmCIF format [Save to disk]

View 1P3H in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1P3H

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1P3H, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1p3ha_, region A [Jmol] [rasmolscript] [script source]     - Domain d1p3hb_, region B [Jmol] [rasmolscript] [script source]     - Domain d1p3hc_, region C [Jmol] [rasmolscript] [script source]     - Domain d1p3hd_, region D [Jmol] [rasmolscript] [script source]     - Domain d1p3he_, region E [Jmol] [rasmolscript] [script source]     - Domain d1p3hf_, region F [Jmol] [rasmolscript] [script source]     - Domain d1p3hg_, region G [Jmol] [rasmolscript] [script source]     - Domain d1p3hh_, region H [Jmol] [rasmolscript] [script source]     - Domain d1p3hi_, region I [Jmol] [rasmolscript] [script source]     - Domain d1p3hj_, region J [Jmol] [rasmolscript] [script source]     - Domain d1p3hk_, region K [Jmol] [rasmolscript] [script source]     - Domain d1p3hl_, region L [Jmol] [rasmolscript] [script source]     - Domain d1p3hm_, region M [Jmol] [rasmolscript] [script source]     - Domain d1p3hn_, region N [Jmol] [rasmolscript] [script source] Fold representative 1p3h from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1p3h_G] [1p3h_D] [1p3h_F] [1p3h_I] [1p3h_A] [1p3h_M] [1p3h_L] [1p3h_H] [1p3h_C] [1p3h_K] [1p3h_N] [1p3h_J] [1p3h_E] [1p3h_B]

Other resources with information on 1P3H

InterPro: IPR001476 MMDB (Entrez's Structure Database)

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