1OSP	Complex (Immunoglobulin Lipoprotein)	date	Nov 23, 1996
title	Crystal Structure Of Outer Surface Protein A Of Borrelia Burgdorferi Complexed With A Murine Monoclonal Antibody Fab
authors	H.Li, C.L.Lawson
compound		source
Molecule: Fab 184.1 Chain: L		Organism_scientific: Mus Musculus Organism_common: Mouse
Molecule: Fab 184.1 Chain: H		Organism_scientific: Mus Musculus Organism_common: Mouse
Molecule: Outer Surface Protein A Chain: O Synonym: Ospa Engineered: Yes Mutation: Yes		Organism_scientific: Borrelia Burgdorferi Organism_common: Lyme Disease Spirochete Strain: B31 Expression_system: Escherichia Coli Expression_system_plasmid: Pet9c
symmetry	Space Group: P 21 21 21	R_factor	0.229
crystal cell	length a length b length c angle alpha angle beta angle gamma 89.100 91.700 100.400 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	1.95 Å
note	1OSP is a representative structure
related structures	by homologous chain: 1MIE, 1Q9K
similarity	Belongs to the Lipoprotein_1 family.
subcellular loc.	Attached to the outer membrane by a lipid anchor.
Gene	BB ; OSPA (B. burgdorferi)
Gene Ontology	Chain Function Process Component O cell outer membrane membrane outer membrane
Primary reference	Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab., Li H, Dunn JJ, Luft BJ, Lawson CL, Proc Natl Acad Sci U S A 1997 Apr 15;94(8):3584-9. PMID:9108020

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (150 Kb) [Save to disk] Biological Unit Coordinates (1osp.pdb1.gz) 146 Kb CSU: Contacts of Structural Units for 1OSP Likely Quarternary Molecular Structure file(s) for 1OSP Retrieve 1OSP in mmCIF format [Save to disk]

View 1OSP in 3D

Proteopedia, because life has more than 2D. On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1OSP

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1OSP, from MSDmotif at EBI Genome occurence of 1OSP's fold from GeneCensus Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1osph1, region H:1-120 [Jmol] [rasmolscript] [script source]     - Domain d1osph2, region H:121-218 [Jmol] [rasmolscript] [script source]     - Domain d1ospl1, region L:1-107 [Jmol] [rasmolscript] [script source]     - Domain d1ospl2, region L:108-214 [Jmol] [rasmolscript] [script source]     - Domain d1ospo_, region O [Jmol] [rasmolscript] [script source] Fold representative 1osp from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1osp_O] [1osp_H] [1osp_L]

Other resources with information on 1OSP

InterPro: IPR001809 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

Movements, Movies and Images

Domain movements in 1OSP from the Database of Macromolecular Movements. Images from IMB Jena Image Library of Biological Macromolecules.

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