1M3J	Toxin	date	Jun 28, 2002
title	Crystal Form II Of Perfringolysin O
authors	J.Rossjohn, M.Parker, G.Polekhina, S.Feil, R.Tweten
compound		source
Molecule: Perfringolysin O Chain: A, B Engineered: Yes		Organism_scientific: Clostridium Perfringens Organism_common: Bacteria Expression_system: Escherichia Coli Expression_system_common: Bacteria
symmetry	Space Group: P 21 21 2	R_factor	0.247
crystal cell	length a length b length c angle alpha angle beta angle gamma 167.000 214.110 47.070 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	3.00 Å
related structures	by homologous chain: 1M3I
similarity	Belongs to the thiol-activated cytolysin family.[Thiol_cytolysin]
subunit	Forms oligomers in the host membrane.
Genes	CPE0163, PFOA, PFOR, PFO (C. perfringens)
function	Cholesterol is the receptor for the binding of these toxins to eukaryotic cell membranes. Is able to lyse cholesterol containing membranes. Sulfhydryl-activated toxin. Can be reversibly inactivated by oxidation.
Gene Ontology	Chain Function Process Component A, B lipid binding cholesterol binding pathogenesis cytolysis hemolysis by symbiont of hos...

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (155 Kb) [Save to disk] Biological Unit Coordinates (1m3j.pdb1.gz) 78 Kb Biological Unit Coordinates (1m3j.pdb2.gz) 76 Kb CSU: Contacts of Structural Units for 1M3J Likely Quarternary Molecular Structure file(s) for 1M3J Retrieve 1M3J in mmCIF format [Save to disk]

View 1M3J in 3D

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Visual 3D analysis of 1M3J

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1M3J, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1m3ja_, region A [Jmol] [rasmolscript] [script source]     - Domain d1m3jb_, region B [Jmol] [rasmolscript] [script source] Fold representative 1m3j from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1m3j_A] [1m3j_B]

Other resources with information on 1M3J

InterPro: IPR001869 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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