1M3I	Toxin	date	Jun 28, 2002
title	Perfringolysin O, New Crystal Form
authors	J.Rossjohn, M.Parker, G.Polekhina, S.Feil, R.Tweten
compound		source
Molecule: Perfringolysin O Chain: A, B, C, D Engineered: Yes		Organism_scientific: Clostridium Perfringens Organism_common: Bacteria Gene: Pfoa Expression_system: Escherichia Coli Expression_system_common: Bacteria
symmetry	Space Group: P 31	R_factor	0.233
crystal cell	length a length b length c angle alpha angle beta angle gamma 130.410 130.410 129.900 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.90 Å
related structures	by homologous chain: 1M3J, 1PFO
similarity	Belongs to the thiol-activated cytolysin family.[Thiol_cytolysin]
subunit	Forms oligomers in the host membrane.
Genes	CPE0163, PFOA, PFOR, PFO (C. perfringens)
function	Cholesterol is the receptor for the binding of these toxins to eukaryotic cell membranes. Is able to lyse cholesterol containing membranes. Sulfhydryl-activated toxin. Can be reversibly inactivated by oxidation.
Gene Ontology	Chain Function Process Component A, D, C, B cholesterol binding pathogenesis

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (303 Kb) [Save to disk] Biological Unit Coordinates (1m3i.pdb1.gz) 83 Kb Biological Unit Coordinates (1m3i.pdb2.gz) 77 Kb Biological Unit Coordinates (1m3i.pdb3.gz) 76 Kb Biological Unit Coordinates (1m3i.pdb4.gz) 76 Kb CSU: Contacts of Structural Units for 1M3I Likely Quarternary Molecular Structure file(s) for 1M3I Retrieve 1M3I in mmCIF format [Save to disk]

View 1M3I in 3D

Proteopedia, because life has more than 2D. On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1M3I

Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only) Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges. Cartoon representation from PDB Cartoon Ramachandran plot from PDBSum

Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1M3I, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1m3ia_, region A [Jmol] [rasmolscript] [script source]     - Domain d1m3ib_, region B [Jmol] [rasmolscript] [script source]     - Domain d1m3ic_, region C [Jmol] [rasmolscript] [script source]     - Domain d1m3id_, region D [Jmol] [rasmolscript] [script source] Fold representative 1m3i from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1m3i_D] [1m3i_C] [1m3i_B] [1m3i_A]

Other resources with information on 1M3I

InterPro: IPR001869 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

Movements, Movies and Images

Images from IMB Jena Image Library of Biological Macromolecules.

You may enter another PDB ID code

Go [Back], to the [PDB Lite page], to the [OCA Search page] or to the [PDB Home page]

---

OCA© by Jaime Prilusky, 1996-2007
Bioinformatics and Biological Computing
Weizmann Institute of Science

---