1L7H	Hydrolase	date	Mar 15, 2002
title	Crystal Structure Of R292k Mutant Influenza Virus Neuraminidase In Complex With Bcx-1812
authors	B.J.Smith, J.L.Mckimm-Breshkin, M.Mcdonald, R.T.Fernley, J.N.Varghese, P.M.Colman
compound		source
Molecule: Neuraminidase Chain: A Fragment: Integral Membrane Protein, Membrane Stalk Cleaved By Pronase Releasing Fully Active Residues 82-468; Ec: 3.2.1.18 Mutation: Yes		Organism_scientific: Influenza A Virus Organism_taxid: 11320 Strain: Anwsternaustraliag70c75
symmetry	Space Group: I 4 3 2	R_factor 0.145 R_Free 0.177
crystal cell	length a length b length c angle alpha angle beta angle gamma 180.831 180.831 180.831 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	1 Å
ligand	BCZ, BMA, CA, GOL, MAN, NAG	enzyme	Exo-alpha-sialidase;. Neuraminidase;. Sialidase;. Alpha-neuraminidase;. Acetylneuraminidase. Hydrolase E.C.3.2.1.18 BRENDA
related structures	by homologous chain: 1L7G, 2QWG
domain	The transmembrane domain also plays a role in lipid raft association (by similarity). Intact n-terminus is essential for virion morphogenesis. Possess two apical sorting signals, one in the ectodomain, which is likely to be a glycan, and the other in the transmembrane domain.
similarity	Belongs to the glycosyl hydrolase 34 family.[Neur]
subunit	Homotetramer (by similarity).
catalytic activ.	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
post-translat. modifications	N-glycosylated (by similarity).
subcellular loc.	Preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. Uses lipid rafts for cell surface transport and apical sorting. Type ii membrane protein. In the virion, forms a mushroom-shaped spike on the surface of the membrane (by similarity).
enzyme regulation	These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Inhibited by the neuraminidase inhibitors zanamivir (relenza) and oseltamivir (tamiflu). Resistance to neuraminidase inhibitors is quite rare.
Gene	NA
function	Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. Otherwise, infection would be limited to one round of replication. Cleaves off the terminal sialic acids on the glycosylated ha during virus budding to facilitate virus release. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication. May additionally display a raft-association independent effect on budding.
Gene Ontology	Chain Function Process Component A exo-alpha-sialidase activity... calcium ion binding hydrolase activity hydrolase activity, acting o... metal ion binding carbohydrate metabolic proce... metabolic process plasma membrane membrane integral to membrane virion host cell plasma membrane virion membrane
Primary reference	Structural studies of the resistance of influenza virus neuramindase to inhibitors., Smith BJ, McKimm-Breshkin JL, McDonald M, Fernley RT, Varghese JN, Colman PM, J Med Chem 2002 May 23;45(11):2207-12. PMID:12014958

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (85 Kb) [Save to disk] Biological Unit Coordinates (1l7h.pdb1.gz) 305 Kb CSU: Contacts of Structural Units for 1L7H Likely Quarternary Molecular Structure file(s) for 1L7H Structure Factors (391 Kb) Retrieve 1L7H in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1L7H, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1l7ha_, region A [Jmol] [rasmolscript] [script source] Fold representative 1l7h from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1l7h_A]

Other resources with information on 1L7H

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