1KLX	Hydrolase	date	Dec 13, 2001
title	Helicobacter Pylori Cysteine Rich Protein B (Hcpb)
authors	L.Luethy, M.G.Gruetter, P.R.E.Mittl
compound		source
Molecule: Cysteine Rich Protein B Chain: A Engineered: Yes		Organism_scientific: Helicobacter Pylori Organism_common: Bacteria Gene: Hp0336 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21(De3) Expression_system_vector_type: Plasmid Expression_system_plasmid: Ptft74
symmetry	Space Group: P 65 2 2	R_factor	0.187
crystal cell	length a length b length c angle alpha angle beta angle gamma 51.360 51.360 205.941 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	1.95 Å
note	1KLX is a representative structure
similarity	Contains3 tpr repeats. Belongs to the hcp beta-lactamase family.
catalytic activ.	A beta-lactam + h(2)o = a substituted beta- amino acid.
gene	hcpB (H. pylori)
function	Hydrolyzes 6-aminopenicillinic acid and 7- aminocephalosporanic acid (aca) derivatives.
Gene Ontology	Chain Function Process Component A binding beta-lactamase activity hydrolase activity response to antibiotic
Primary reference	The crystal structure of Helicobacter pylori cysteine-rich protein B reveals a novel fold for a penicillin-binding protein., Luthy L, Grutter MG, Mittl PR, J Biol Chem 2002 Mar 22;277(12):10187-93. PMID:11777911

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (35 Kb) [Save to disk] Biological Unit Coordinates (1klx.pdb1.gz) 25 Kb CSU: Contacts of Structural Units for 1KLX Likely Quarternary Molecular Structure file(s) for 1KLX Structure Factors (130 Kb) Retrieve 1KLX in mmCIF format [Save to disk]

View 1KLX in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1KLX

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1KLX, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1klxa_, region A [Jmol] [rasmolscript] [script source] Fold representative 1klx from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1klx_A]

Other resources with information on 1KLX

InterPro: IPR001440 , IPR011990 , IPR006597 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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