1KHO | Toxin | date | Nov 30, 2001 ![]() ![]() |
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title | Crystal Structure Analysis Of Clostridium Perfringens Alpha- Toxin Isolated From Avian Strain Swcp | ||||||||||||||
authors | N.Justin, D.S.Moss, R.W.Titball, A.K.Basak | ||||||||||||||
compound | source | ||||||||||||||
Molecule: Alpha-Toxin Chain: A, B Engineered: Yes |
Organism_scientific: Clostridium Perfringens Organism_common: Bacteria Gene: Cpa Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 | ||||||||||||||
symmetry | Space Group: P 41 2 2 | R_factor | 0.210 | ||||||||||||
crystal cell |
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method | X-Ray Diffraction | resolution | 2.40 Å | ||||||||||||
ligand | ZN | enzyme |
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related structures | by homologous chain: 1OLP, 1QM6, 1QMD | ||||||||||||||
domain | The c-terminal domain is a putative phospholipid-recognition domain, which shows structural homology with phospholipid-binding c2-like domains from a range of eukaryotic proteins. The protein is composed of 2 domains; the n-terminal domain contains the phospholipase c active site (plc), in a cleft which is also occupied by the 3 zinc ions. The ability to bind membrane phospholipids in a ca(2+) dependent manner and toxicity is conferred by this c-terminal domain, which also contributes to the sphingomyelinase activity. | ||||||||||||||
similarity | Contains 1 plat domain.[Zn_dep_PLPC] | ||||||||||||||
catalytic activ. | A phosphatidylcholine + h(2)o = 1,2- diacylglycerol + choline phosphate. | ||||||||||||||
subcellular loc. |
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Genes | CPA, PLC (C. perfringens) | ||||||||||||||
function | Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin, causing cell rupture. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase c, leading to activation of eukaryotic phospholipases and further membrane damage. Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. | ||||||||||||||
Gene Ontology |
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Primary reference | The first strain of Clostridium perfringens isolated from an avian source has an alpha-toxin with divergent structural and kinetic properties., Justin N, Walker N, Bullifent HL, Songer G, Bueschel DM, Jost H, Naylor C, Miller J, Moss DS, Titball RW, Basak AK, Biochemistry 2002 May 21;41(20):6253-62. PMID:12009886 |
Data retrieval |
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View 1KHO in 3D |
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Visual 3D analysis of 1KHO |
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Structure-derived information |
- Domain d1khoa1, region A:1-249 [Jmol] [rasmolscript] [script source] - Domain d1khoa2, region A:250-370 [Jmol] [rasmolscript] [script source] - Domain d1khob1, region B:1-249 [Jmol] [rasmolscript] [script source] - Domain d1khob2, region B:250-370 [Jmol] [rasmolscript] [script source] |
Sequence-derived information |
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