1I4G	Toxin	date	Feb 21, 2001
title	Crystal Structure Of Staphylococcal Enterotoxin A Mutant H187a With Reduced Zn2+ Affinity
authors	M.Hakansson, P.Antonsson, P.Bjork, L.A.Svensson
compound		source
Molecule: Enterotoxin Type A Chain: A, B Synonym: Sea Engineered: Yes Mutation: Yes		Organism_scientific: Staphylococcus Aureus Organism_common: Bacteria Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Ul635 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pkp889
symmetry	Space Group: P 21 21 21	R_factor	0.178
crystal cell	length a length b length c angle alpha angle beta angle gamma 73.660 84.830 86.350 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.10 Å
ligand	SO4, ZN	enzyme
related structures	by homologous chain: 1ESF, 1GXS, 1SXT
similarity	Belongs to the staphylococcal/streptococcal toxin family.[Stap_Strp_tox_C]
subunit	Monomer.
subcellular loc.	Secreted protein.
Gene	ENTA (S. aureus)
function	The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death. Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome.
Gene Ontology	Chain Function Process Component A, B zinc ion binding metal ion binding pathogenesis extracellular region
Primary reference	Cooperative zinc binding in a staphylococcal enterotoxin A mutant mimics the SEA-MHC class II interaction., Hakansson M, Antonsson P, Bjork P, Svensson LA, J Biol Inorg Chem 2001 Oct;6(8):757-62. PMID:11713682

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (83 Kb) [Save to disk] Biological Unit Coordinates (1i4g.pdb1.gz) 43 Kb Biological Unit Coordinates (1i4g.pdb2.gz) 39 Kb CSU: Contacts of Structural Units for 1I4G Likely Quarternary Molecular Structure file(s) for 1I4G Retrieve 1I4G in mmCIF format [Save to disk]

View 1I4G in 3D

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1I4G, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1i4ga1, region A:10-120 [Jmol] [rasmolscript] [script source]     - Domain d1i4ga2, region A:121-233 [Jmol] [rasmolscript] [script source]     - Domain d1i4gb2, region B:121-233 [Jmol] [rasmolscript] [script source]     - Domain d1i4gb1, region B:8-120 [Jmol] [rasmolscript] [script source] Fold representative 1i4g from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1i4g_B] [1i4g_A]

Other resources with information on 1I4G

InterPro: IPR006123 , IPR006173 , IPR006177 , IPR006126 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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