1GN4 Oxidoreductase date Oct 02, 2001
title H145e Mutant Of Mycobacterium Tuberculosis Iron-Superoxide Dismutase.
authors K.A.Bunting, J.B.Cooper, M.O.Badasso, I.J.Tickle, M.Newton, S.P.Wood, Y.Zhang, D.B.Young
compound source
Molecule: Superoxide Dismutase
Chain: A,B,C,D
Ec: 1.15.1.1
Engineered: Yes
Mutation: Yes
 Organism_scientific: Mycobacterium Tuberculosis
Expression_system: Mycobacterium Vaccae
symmetry Space Group: P 21 21 21
R_factor 0.172
crystal
cell
length a length b length c angle alpha angle beta angle gamma
107.660 102.860 73.940 90.00 90.00 90.00
method X-Ray Diffractionresolution 2.5 Å
ligand MN enzyme Superoxide dismutase;. Superoxidase dismutase;. Copper-zinc superoxide dismutase;. Cu-Zn superoxide dismutase;. Ferrisuperoxide dismutase;. Superoxide dismutase I;. Superoxide dismutase II;. SOD;. Cu,Zn-SOD;. Mn-SOD;. Fe-SOD;. SODF;. SODS;. SOD-1;. SOD-2;. SOD-3;. SOD-4;. Hemocuprein;. Erythrocuprein;. Cytocuprein;. Cuprein;. Hepatocuprein. Oxidoreductase E.C.1.15.1.1 BRENDA
similarity Belongs to the iron/manganese superoxide dismutase family.[Sod_Fe_C]
subunit Homotetramer.
catalytic activ. 2 superoxide + 2 h(+) = o(2) + h(2)o(2).
subcellular loc. Secreted protein.
genes sodB (A. fumigatus); SOD1 (B. taurus); sodA (D. radiodurans); sodA, sodB, sodC (E. coli); SOD1, SOD2 (H. sapiens); sod (M. thermoautotrophicum); MT0447, sodC (M. tuberculosis); sodC (P. leiognathi); sodB (P. gingivalis); sodA (P. freudenreichii); sodB (P. putida); sod (P. aerophilum); sodC1, sodC (S. typhimurium); SOD (S. mansoni); SODCP (S. oleracea); sodN (S. seoulensis); sod (S. acidocaldarius); sod (S. solfataricus); sodA (T. thermophilus); sod1-B (X. laevis); SOD1 (S. cerevisiae)
function Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Gene
Ontology
ChainFunctionProcessComponent
A, D, C, B
  • superoxide dismutase activit...
  • iron ion binding
  • oxidoreductase activity
  • metal ion binding
  • superoxide metabolic process...

    • disease Amyotrophic lateral sclerosis,due to SOD1 deficiency
    Superoxide Dismutase 2; Sod2
    Primary referenceEngineering a change in metal-ion specificity of the iron-dependent superoxide dismutase from Mycobacterium tuberculosis-- X-ray structure analysis of site-directed mutants., Bunting K, Cooper JB, Badasso MO, Tickle IJ, Newton M, Wood SP, Zhang Y, Young D, Eur J Biochem 1998 Feb 1;251(3):795-803. PMID:9490054
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (154 Kb) [Save to disk]
  • Biological Unit Coordinates (1gn4.pdb1.gz) 133 Kb
  • CSU: Contacts of Structural Units for 1GN4
  • Likely Quarternary Molecular Structure file(s) for 1GN4
  • Structure Factors (364 Kb)
  • Retrieve 1GN4 in mmCIF format [Save to disk]
    • View 1GN4 in 3D
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
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  • 3D motif for 1GN4, from MSDmotif at EBI
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1gn4a1, region A:2-85 [Jmol] [rasmolscript] [script source]
        - Domain d1gn4a2, region A:86-199 [Jmol] [rasmolscript] [script source]
        - Domain d1gn4b1, region B:2-85 [Jmol] [rasmolscript] [script source]
        - Domain d1gn4b2, region B:86-199 [Jmol] [rasmolscript] [script source]
        - Domain d1gn4c1, region C:2-85 [Jmol] [rasmolscript] [script source]
        - Domain d1gn4c2, region C:86-199 [Jmol] [rasmolscript] [script source]
        - Domain d1gn4d1, region D:2-85 [Jmol] [rasmolscript] [script source]
        - Domain d1gn4d2, region D:86-199 [Jmol] [rasmolscript] [script source]
  • Fold representative 1gn4 from FSSP and Dali (Families of Structurally Similar Proteins)
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1gn4_A] [1gn4_B] [1gn4_C] [1gn4_D]
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  • InterPro: IPR001189
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