1G8Z	Toxin	date	Nov 21, 2000
title	His57ala Mutant Of Cholera Toxin B-Penatmer
authors	A.T.Aman, S.Fraser, E.A.Merritt, C.Rodigherio, M.Kenny
compound		source
Molecule: Cholera Toxin B Protein Chain: D, E, F, G, H Synonym: Ctb Engineered: Yes Mutation: Yes		Organism_scientific: Vibrio Cholerae Organism_common: Bacteria Gene: Ctxb Expression_system: Escherichia Coli Expression_system_common: Bacteria
symmetry	Space Group: P 21 21 2	R_factor	0.179
crystal cell	length a length b length c angle alpha angle beta angle gamma 101.362 114.722 45.591 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.00 Å
ligand	GAL	enzyme
similarity	Belongs to the Enterotoxin_b family.
subunit	The a subunit contains two chains, a1 and a2, linked by a disulfide bridge. The holotoxin (choleragen) consists of a pentameric ring of b subunits whose central pore is occupied by the a subunit.
gene	ctxB (V. cholerae)
function	It can bind five gm1 gangliosides. The b subunit pentameric ring directs the a subunit to its target by binding to the gm1 gangliosides present on the surface of the intestinal epithelial cells. It has no toxic activity by itself.
Gene Ontology	Chain Function Process Component H, F, D, G, E pathogenesis extracellular region membrane
Primary reference	A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity., Aman AT, Fraser S, Merritt EA, Rodigherio C, Kenny M, Ahn M, Hol WG, Williams NA, Lencer WI, Hirst TR, Proc Natl Acad Sci U S A 2001 Jul 17;98(15):8536-41. PMID:11447291

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (105 Kb) [Save to disk] Biological Unit Coordinates (1g8z.pdb1.gz) 92 Kb CSU: Contacts of Structural Units for 1G8Z Likely Quarternary Molecular Structure file(s) for 1G8Z Structure Factors (521 Kb) Retrieve 1G8Z in mmCIF format [Save to disk]

View 1G8Z in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1G8Z

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1G8Z, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1g8zd_, region D [Jmol] [rasmolscript] [script source]     - Domain d1g8ze_, region E [Jmol] [rasmolscript] [script source]     - Domain d1g8zf_, region F [Jmol] [rasmolscript] [script source]     - Domain d1g8zg_, region G [Jmol] [rasmolscript] [script source]     - Domain d1g8zh_, region H [Jmol] [rasmolscript] [script source] Fold representative 1g8z from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1g8z_E] [1g8z_D] [1g8z_F] [1g8z_G] [1g8z_H]

Other resources with information on 1G8Z

InterPro: IPR001835 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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