1FV3 | Toxin | date | Sep 18, 2000 ![]() ![]() |
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title | The Hc Fragment Of Tetanus Toxin Complexed With An Analogue Of Its Ganglioside Receptor Gt1b | ||||||||||||||
authors | C.Fotinou, P.Emsley, I.Black, H.Ando, H.Ishida, M.Kiso, K.A.Sinha, N.F.Fairweather, N.W.Isaacs | ||||||||||||||
compound | source | ||||||||||||||
Molecule: Tetanus Toxin Heavy Chain Chain: A, B Fragment: C-Terminal Domain Of Heavy Chain Engineered: Yes |
Organism_scientific: Clostridium Tetani Organism_common: Bacteria Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_plasmid: Pet28 | ||||||||||||||
symmetry | Space Group: P 1 21 1 | R_factor | 0.237 | ||||||||||||
crystal cell |
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method | X-Ray Diffraction | resolution | 2.30 Å | ||||||||||||
ligand | BGC, CEQ, GAL, NGA, PO4, SIA, SLB | enzyme |
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related structures | by homologous chain: 1YXW, 1YYN | ||||||||||||||
similarity | Belongs to the peptidase m27 family.[Toxin_trans] | ||||||||||||||
subunit | The precursor polypeptide is subsequently cleaved to yield subchains l and h. These remain linked by a disulfide bridge and are non-toxic after separation. | ||||||||||||||
catalytic activ. | Hydrolysis of 76-gln-|-phe-77 bond in synaptobrevin 2. | ||||||||||||||
Gene | CTC ; TETX (C. tetani) | ||||||||||||||
function | It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. Tetanus toxin acts by inhibiting neurotransmitter release. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-gln-|-phe-77 bond of synaptobrevin-2. | ||||||||||||||
Gene Ontology | |||||||||||||||
Primary reference | The crystal structure of tetanus toxin Hc fragment complexed with a synthetic GT1b analogue suggests cross-linking between ganglioside receptors and the toxin., Fotinou C, Emsley P, Black I, Ando H, Ishida H, Kiso M, Sinha KA, Fairweather NF, Isaacs NW, J Biol Chem 2001 Aug 24;276(34):32274-81. PMID:11418600 |
Data retrieval |
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Visual 3D analysis of 1FV3 |
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Structure-derived information |
- Domain d1fv3a2, region A:1111-1315 [Jmol] [rasmolscript] [script source] - Domain d1fv3a1, region A:865-1110 [Jmol] [rasmolscript] [script source] - Domain d1fv3b2, region B:1111-1315 [Jmol] [rasmolscript] [script source] - Domain d1fv3b1, region B:865-1110 [Jmol] [rasmolscript] [script source] |
Sequence-derived information |
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