1FGB	Enterotoxin	date	Feb 21, 1996
title	Toxin
authors	R.-G.Zhang, E.Westbrook
compound		source
Molecule: Cholera Toxin B Subunit Pentamer Chain: D, E, F, G, H Synonym: Choleragenoid		Organism_scientific: Vibrio Cholerae Strain: 569b
symmetry	Space Group: P 1 21 1	R_factor	0.171
crystal cell	length a length b length c angle alpha angle beta angle gamma 39.100 94.500 67.600 90.00 96.10 90.00
method	X-Ray Diffraction	resolution	2.4 Å
similarity	Belongs to the Enterotoxin_b family.
subunit	The a subunit contains two chains, a1 and a2, linked by a disulfide bridge. The holotoxin (choleragen) consists of a pentameric ring of b subunits whose central pore is occupied by the a subunit.
gene	ctxB (V. cholerae)
function	It can bind five gm1 gangliosides. The b subunit pentameric ring directs the a subunit to its target by binding to the gm1 gangliosides present on the surface of the intestinal epithelial cells. It has no toxic activity by itself.
Gene Ontology	Chain Function Process Component H, F, D, G, E pathogenesis extracellular region membrane
Primary reference	The 2.4 A crystal structure of cholera toxin B subunit pentamer: choleragenoid., Zhang RG, Westbrook ML, Westbrook EM, Scott DL, Otwinowski Z, Maulik PR, Reed RA, Shipley GG, J Mol Biol 1995 Aug 25;251(4):550-62. PMID:7658472

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (97 Kb) [Save to disk] Biological Unit Coordinates (1fgb.pdb1.gz) 85 Kb CSU: Contacts of Structural Units for 1FGB Likely Quarternary Molecular Structure file(s) for 1FGB Retrieve 1FGB in mmCIF format [Save to disk]

View 1FGB in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1FGB

Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only) Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges. STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure. GRASS (Graphical Representation and Analysis of Structure Server) Cartoon representation from PDB Cartoon Ramachandran plot from PDBSum

Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1FGB, from MSDmotif at EBI Genome occurence of 1FGB's fold from GeneCensus Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1fgbd_, region D [Jmol] [rasmolscript] [script source]     - Domain d1fgbe_, region E [Jmol] [rasmolscript] [script source]     - Domain d1fgbf_, region F [Jmol] [rasmolscript] [script source]     - Domain d1fgbg_, region G [Jmol] [rasmolscript] [script source]     - Domain d1fgbh_, region H [Jmol] [rasmolscript] [script source] Fold representative 1fgb from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1fgb_G] [1fgb_D] [1fgb_H] [1fgb_E] [1fgb_F]

Other resources with information on 1FGB

InterPro: IPR001835 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

Movements, Movies and Images

Images from IMB Jena Image Library of Biological Macromolecules.

You may enter another PDB ID code

Go [Back], to the [PDB Lite page], to the [OCA Search page] or to the [PDB Home page]

---

OCA© by Jaime Prilusky, 1996-2006
Bioinformatics and Biological Computing
Weizmann Institute of Science

---