1F0P	Transferase	date	May 16, 2000
title	Mycobacterium Tuberculosis Antigen 85b With Trehalose
authors	D.H.Anderson, G.Harth, M.A.Horwitz, D.Eisenberg, Tb Structural Genomics Consortium (Tbsgc)
compound		source
Molecule: Antigen 85-B Chain: A Engineered: Yes		Organism_scientific: Mycobacterium Tuberculosis Organism_common: Bacteria Expression_system: Mycobacterium Smegmatis Expression_system_common: Bacteria Expression_system_plasmid: Psmt3
symmetry	Space Group: P 31 2 1	R_factor
crystal cell	length a length b length c angle alpha angle beta angle gamma 73.210 73.210 92.544 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	1.90 Å
ligand	MES, MPD, TRE	enzyme
similarity	Belongs to the mycobacterial a85 antigen family.[Esterase]
subcellular loc.	Secreted protein.
genes	MT1934, fbpB (M. tuberculosis)
function	Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.
Gene Ontology	Chain Function Process Component A acyltransferase activity transferase activity
Primary reference	An interfacial mechanism and a class of inhibitors inferred from two crystal structures of the Mycobacterium tuberculosis 30 kDa major secretory protein (Antigen 85B), a mycolyl transferase., Anderson DH, Harth G, Horwitz MA, Eisenberg D, J Mol Biol 2001 Mar 23;307(2):671-81. PMID:11254389

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (63 Kb) [Save to disk] Biological Unit Coordinates (1f0p.pdb1.gz) 51 Kb CSU: Contacts of Structural Units for 1F0P Likely Quarternary Molecular Structure file(s) for 1F0P Structure Factors (272 Kb) Retrieve 1F0P in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1F0P, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1f0pa_, region A [Jmol] [rasmolscript] [script source] Fold representative 1f0p from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1f0p_A]

Other resources with information on 1F0P

InterPro: IPR000801 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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