1ENZ	Oxidoreductase	date	Jan 27, 1995
authors	A.Dessen, A.Quemard, J.S.Blanchard, W.R.Jacobs Jr., J.C.Sacchettini, Tb Structural Genomics Consortium (Tbsgc)
compound		source
Molecule: Enoyl-Acyl Carrier Protein (Acp) Reductase Chain: Null Synonym: Inha Engineered: Yes Mutation: S94a		Organism_scientific: Mycobacterium Tuberculosis Expression_system: Escherichia Coli
symmetry	Space Group: P 62 2 2	R_factor	0.193
crystal cell	length a length b length c angle alpha angle beta angle gamma 100.140 100.140 139.950 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.7 Å
ligand	NAD	enzyme
similarity	Fabisubfamily. Belongs to the short-chain dehydrogenases/reductases (sdr) family.
subunit	Homotetramer.
catalytic activ.	Acyl-[acyl-carrier protein] + nad(+) = trans- 2,3-dehydroacyl-[acyl-carrier protein] + nadh.
pathway	Second reductive step in fatty acid biosynthesis. This isozyme is involved in mycolic acid biosynthesis.
genes	MT1531, inhA (M. tuberculosis)
function	Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.
Gene Ontology	Chain Function Process Component A enoyl-[acyl-carrier-protein]... oxidoreductase activity fatty acid biosynthetic proc... metabolic process lipid biosynthetic process response to antibiotic
Primary reference	Crystal structure and function of the isoniazid target of Mycobacterium tuberculosis., Dessen A, Quemard A, Blanchard JS, Jacobs WR Jr, Sacchettini JC, Science 1995 Mar 17;267(5204):1638-41. PMID:7886450

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (53 Kb) [Save to disk] Biological Unit Coordinates (1enz.pdb1.gz) 82 Kb CSU: Contacts of Structural Units for 1ENZ Likely Quarternary Molecular Structure file(s) for 1ENZ Retrieve 1ENZ in mmCIF format [Save to disk]

View 1ENZ in 3D

On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 1ENZ

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1ENZ, from MSDmotif at EBI Genome occurence of 1ENZ's fold from GeneCensus Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1enz__, region [Jmol] [rasmolscript] [script source] Fold representative 1enz from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1enz_]

Other resources with information on 1ENZ

InterPro: IPR002347 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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