1E9Y	Hydrolase	date	Nov 01, 2000
title	Crystal Structure Of Helicobacter Pylori Urease In Complex With Acetohydroxamic Acid
authors	N.-C.Ha, S.-T.Oh, B.-H.Oh
compound		source
Molecule: Urease Alpha Subunit Chain: A Engineered: Yes Ec: 3.5.1.5		Organism_scientific: Helicobacter Pylori Expression_system: Escherichia Coli
Molecule: Urease Beta Subunit Chain: B Engineered: Yes		Organism_scientific: Helicobacter Pylori Expression_system: Escherichia Coli
symmetry	Space Group: I 2 3	R_factor	0.213
crystal cell	length a length b length c angle alpha angle beta angle gamma 178.160 178.160 178.160 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	3.0 Å
ligand	HAE, KCX, NI	enzyme	Urease. Hydrolase E.C.3.5.1.5 BRENDA
similarity	In the n-terminal section; belongs to the urease gamma subunit family. Belongs to the urease family. In the c-terminal section; belongs to the urease beta subunit family.[Amidohydro_1]
subunit	Heterohexamer of 3 alpha and 3 beta subunits. Four heterohexamers assemble to form a 16 nm spherical complex.
catalytic activ.	Urea + h(2)o = co(2) + 2 nh(3).
post-translat. modifications	Lys-219 is carbamylated. The carbamoyl group provides the ligands for the two nickel ions (by similarity).
subcellular loc.	Cytoplasm.
genes	ureA, ureB, ureC (B. pasteurii); ureA, ureB (H. pylori); ureA, ureB, ureC (K. aerogenes)
Gene Ontology	Chain Function Process Component A urease activity nickel ion binding hydrolase activity nitrogen compound metabolic ... cytoplasm B urease activity nickel ion binding hydrolase activity metal ion binding nitrogen compound metabolic ... urea metabolic process
Primary reference	Supramolecular assembly and acid resistance of Helicobacter pylori urease., Ha NC, Oh ST, Sung JY, Cha KA, Lee MH, Oh BH, Nat Struct Biol 2001 Jun;8(6):505-9. PMID:11373617

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (149 Kb) [Save to disk] Biological Unit Coordinates (1e9y.pdb1.gz) 1491 Kb CSU: Contacts of Structural Units for 1E9Y Likely Quarternary Molecular Structure file(s) for 1E9Y Retrieve 1E9Y in mmCIF format [Save to disk]

View 1E9Y in 3D

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Visual 3D analysis of 1E9Y

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1E9Y, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1e9ya2, region A:1-105 [Jmol] [rasmolscript] [script source]     - Domain d1e9ya1, region A:106-238 [Jmol] [rasmolscript] [script source]     - Domain d1e9yb1, region B:1-131,B:432-480 [Jmol] [rasmolscript] [script source]     - Domain d1e9yb2, region B:132-431,B:481-569 [Jmol] [rasmolscript] [script source] Fold representative 1e9y from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1e9y_A] [1e9y_B]

Other resources with information on 1E9Y

InterPro: IPR002019 , IPR005848 , IPR008223 , IPR006680 , IPR008295 , IPR002026 , IPR011612 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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