1DTO	Viral Protein	date	Jan 13, 2000
title	Crystal Structure Of The Complete Transactivation Domain Of E2 Protein From The Human Papillomavirus Type 16
authors	A.A.Antson, J.E.Burns, O.V.Moroz, D.J.Scott, C.M.Sanders, I.B.Bronstein, G.G.Dodson, K.S.Wilson, N.Maitland
compound		source
Molecule: Regulatory Protein E2 Chain: A Fragment: Transactivation Domain Engineered: Yes		Organism_scientific: Human Papillomavirus Type 16 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_plasmid: Pet15b
symmetry	Space Group: P 31 2 1	R_factor	0.232
crystal cell	length a length b length c angle alpha angle beta angle gamma 54.680 54.680 155.730 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	1.90 Å
note	1DTO is a representative structure
similarity	Belongs to the papillomaviruses e2 protein family.[PPV_E2_C]
subunit	Binds dna as a dimer.
subcellular loc.	Nucleus.
Gene	E2 (H. papillomavirus)
function	The e1-e2 complex binds to the origin of dna replication. It can either activate or repress transcription depending on e2re's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. E2 regulates viral transcription and dna replication. It binds to the e2re response element (5'-accnnnnnnggt-3') present in multiple copies in the regulatory region.
Gene Ontology	Chain Function Process Component A nucleotide binding DNA binding transcription factor activit... DNA replication regulation of DNA replicatio... transcription regulation of transcription,... viral reproduction nucleus host cell nucleus
Primary reference	Structure of the intact transactivation domain of the human papillomavirus E2 protein., Antson AA, Burns JE, Moroz OV, Scott DJ, Sanders CM, Bronstein IB, Dodson GG, Wilson KS, Maitland NJ, Nature 2000 Feb 17;403(6771):805-9. PMID:10693813

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (42 Kb) [Save to disk] Biological Unit Coordinates (1dto.pdb1.gz) 75 Kb CSU: Contacts of Structural Units for 1DTO Likely Quarternary Molecular Structure file(s) for 1DTO Structure Factors (180 Kb) Retrieve 1DTO in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1DTO, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1dtoa_, region A [Jmol] [rasmolscript] [script source] Fold representative 1dto from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1dto_A]

Other resources with information on 1DTO

InterPro: IPR001866 , IPR012677 , IPR000427 MMDB (Entrez's Structure Database)

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