1DG4	Chaperone	date	Nov 23, 1999
title	Nmr Structure Of The Substrate Binding Domain Of Dnak In The Apo Form
authors	M.Pellecchia, D.L.Montgomery, S.Y.Stevens, C.W.Van Der Kooi, H.Feng, L.M.Gierasch, E.R.P.Zuiderweg
compound		source
Molecule: Dnak Chain: A Fragment: Substrate Binding Domain Other_details: Molecular Chaperone		Organism_scientific: Escherichia Coli Organism_common: Bacteria
method	NMR, 20 Structures
similarity	Belongs to the heat shock protein 70 family.[HSP70]
post-translat. modifications	Autophosphorylated; grpe inhibits the autophosphorylation.
subcellular loc.	Cytoplasmic; has also been isolated in association with the inner membrane.
genes	dnaK, grpF, seg (E. coli)
function	Also participates actively in the response to hyperosmotic shock. Plays an essential role in the initiation of phage lambda dna replication, where it acts in an atp-dependent fashion with the dnaj protein to release lambda o and p proteins from the preprimosomal complex. Dnak is also involved in chromosomal dna replication, possibly through an analogous interaction with the dnaa protein.
Gene Ontology	Chain Function Process Component A nucleotide binding ATP binding unfolded protein binding DNA replication protein folding response to unfolded protein...
Primary reference	Structural insights into substrate binding by the molecular chaperone DnaK., Pellecchia M, Montgomery DL, Stevens SY, Vander Kooi CW, Feng HP, Gierasch LM, Zuiderweg ER, Nat Struct Biol 2000 Apr;7(4):298-303. PMID:10742174

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (542 Kb) [Save to disk] Biological Unit Coordinates (1dg4.pdb1.gz) 552 Kb CSU: Contacts of Structural Units for 1DG4 Retrieve 1DG4 in mmCIF format [Save to disk]

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1DG4, from MSDmotif at EBI Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1dg4a_, region A [Jmol] [rasmolscript] [script source] Fold representative 1dg4 from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1dg4_A]

Other resources with information on 1DG4

InterPro: IPR012725 , IPR001023 NMR Ensemble (OLDERADO: core, domain and representative structure database) PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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