Ab_AFFI 1.0 DOCUMENTATION



Computer program Ab_affi developed for calculating the affinity of monoclonal antibody from non-comptetitive ELISA data. The Ab_affi is a menu-driven computer program for calculating the affinity of monoclonal antibody from non-competitive ELISA data, has been written in GW-BASIC. OD data obtained from the Microplate reader can be fed to computer either directly by interfacing or by using Keyboard and data could be stored for future use. Before starting the calculation, a `dilution template' should be defined and the information about the serial dilutions of antibody added to the wells and antigen coated on the plate must also be supplied. The dilution template consists of arrays of dilution factor (DF), corresponding to the given [Ag] coated on the assay plate. It calculates the average OD of samples, which are in duplicate, triplicate, etc.



The computer program, 1) Fits the OD data for given Ag concentration by hyperbolic curve-fitting method and calculates OD-100, OD-50 and also hyperbolic interpolation formula; 2) It depicts the Sl-range and calculates the linear interpolation formula; and 3.) Checks whether OD-50 is in Sl-range and then calculates accordingly the concentration of [Ab] for a given [Ag] concentration coated on the plate using equ.~(13) and equ.~(15). In a similar way the program calculates the [Ab] concentration at OD-50 for other concentrations of [Ag] coated on the plate. Lastly, it calculates the antibody affinity by using equ.~(11). It also calculates the capacity and flatness of hyperbolic curve, which plays a vital role in ELISA optimization. The program also allows the display and printing of the results. Fig.~1 shows an example printout from one of such ELISA plate.

Files contained in this package:
FILES DESCRIPTION
AB_AFFI.BAS SOURCE CODE OF AB_AFFI, IN GW-BASIC
OD.DAT EXAMPLE FILE CONTAIN OPTICAL DENSITY DATA
DT.DAT EXAMPLE FILE CONTAIN DILUTION TEMPLATE DATA
PAPER.TEX MANUSCRIPT IN TEX FORMAT (NON-DOCUMENT) FILE
abaffi.tar All files in tar format
abaffi.tar.uue uuencoded abaffi.tar file
LICENCE:

This program remains the property of the Institute of Microbial Technology, Chandigarh, INDIA. This program may be freely used by anybody subject to the following conditions:

1. The authors nor the Institute of Microbial Technology Chandigarh assume any responsibility for any losses or damage that may be caused by the use or misuse of the accompanying software.

2. The authors nor the Institute of Microbial Technology Chandigarh give any warranty with regards to the software being able to function on any computer.

3. The accompanying software may not be copied nor distributed with any modifications, and this document file MUST be included with all copies.

4. No fee may be charged for the copying and/or distribution of the accompanying software.

5. Users must agree to accept any risk as a condition of the free use of the accompanying software.

Any suggestion, bug report or comment will be greatly appreciated. Please send them to: 
               G P S Raghava, Scientist 
               Co-ordinator, Bioinformatics Center
               Institute of Microbial Technology, 
               Sector 39A, Chandigarh, India.
               Email address: raghava@imtech.ernet.in