PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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PolysacDB ID	1009
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycolipid
Microbe Name	Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Heat killed Mycobacterium tuberculosis
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 9d8
Antibody Type Class	IgG3
Assay System	Indirect immunofluorescence, ELISA, western blotting
Cross Reactivity	This antibody cross-reacted with M.gordonae, M. gastri, and M. Kansasii
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody has been used in the detection of mycobacteria in clinical samples
Curator ID	AA + AS
Date of Curation	03-01-2010
References	PMC229406

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PolysacDB ID	1090
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Entamoeba histolytica (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Plasma membrane and whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 2D7.10
Antibody Type Class	IgG2b
Assay System	ELISA, immunoblotting
Cross Reactivity	This antibody Was specific to Entamoeba histolytica and did not cross-react with the other Entamoeba isolates
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab would help in the study of carbohydrate antigens that may have relevance in the studies on the mechanism of pathogenicity of the microbe.
Curator ID	AA + AS
Date of Curation	27-01-2010
References	PMC313678

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PolysacDB ID	1105
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalin-fixed A. pleuropneumoniae serotype 4 cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 6F7
Antibody Type Class	IgM
Assay System	Whole cell ELISA and slide agglutination tests
Cross Reactivity	This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7
Curator ID	AA + AS
Date of Curation	31-01-2010
References	PMC170200

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PolysacDB ID	1152
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells of a B. fragilis NCTC 9343 enriched for expression of the EDL
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab QUBF 12
Antibody Type Class	IgG1
Assay System	Immunoblotting, immunofluorescence, flow cytometry
Cross Reactivity	This Mab cross-reacted with B. vulgatus, B. thetawtaomicron, B. ovatus, B. distasonis, B. gingivalis, E. coli or S. aureus. All reacted with B. fragilis strain BE4
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may help in the elucidation of variability of surface polysaccharide structures
Curator ID	AA + AS
Date of Curation	18-02-2010
References	PMC96751

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PolysacDB ID	1264
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans Serotype A strain VW.32 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	UV-killed C. albicans strain VW.32 germ tubes
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3b7
Antibody Type Class	IgM
Assay System	ELISA, Immunofluorescence
Cross Reactivity	This antibody cross-reacted with Candida albicans strain 3153A, strain 996B; Candida tropicalis strain Ct V. did not cross-react with other Candida species such as Candida glabrata, Candida albicans strain 995A and strain L; Candida pseudotropicalis, Candida krusei, Candida parapsilosis
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may help in studying antigens that are involved in pathogenocity
Curator ID	AA + AS
Date of Curation	02-05-2010
References	3058980

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PolysacDB ID	1265
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab C6
Antibody Type Class	IgM
Assay System	ELISA, immunoelecton microscopy and agglutination tests
Cross Reactivity	This antibody cross-reacted with 30 strains of Candida albicans strains, 2 strains of Candida tropicalis but did not cross-react with Candida paratropicalis, Candida parapsilosis, T. glabrata and S. cerevisae.
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab had a agglutinating activity against Candida albicans
Curator ID	AA + AS
Date of Curation	02-05-2010
References	3280163

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PolysacDB ID	1266
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Fractions enriched for mitochondria and other membranous vesicles [membranous fraction] of Candida albicans
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 10G
Antibody Type Class	IgM
Assay System	ELISA, immunocollidal gold electron microscopy, indirect immunofuorescence assay, immunoelectrophoresis
Cross Reactivity	Yeast cells of 15 additional C. albicans strains and isolates of C. stellatoidka, C. tropicalis, C. intermedia and C. lusitaniae were also agglutinated by mAb 1OG. The antigen was not detected on other fungi, including Can&& krusei, C.utilis, Cryptococcus neoformans, Cr. albidus, Torulopsis glabrata, Rhodotorula spp. and Saccharomyces cerevisiae
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab had a agglutinating activity against Candida albicans
Curator ID	AA + AS
Date of Curation	03-05-2010
References	3280163

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PolysacDB ID	1327
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans B311 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 5B2
Antibody Type Class	IgM
Assay System	ELISA, immunofluorescence, crossed immunoelectrophoresis
Cross Reactivity	This antibody cross-reacted with 5 strains of Candida albicans and also cross-reacted with Candida tropicalis and Candida stellatoidia but did not cross-react with T. glabrata and S. cerevisiae
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to isolate surface components and determine the chemical structures that are responsible for the immunological differences. This Mab would facilitate the development of serological assays
Curator ID	AA + AS
Date of Curation	13-06-2010
References	PMC259333

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PolysacDB ID	1329
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans B311 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polyclonal Antisera
Antibody Type Class	IgM
Assay System	ELISA, immunofluorescence, crossed immunoelectrophoresis
Cross Reactivity	This antibody cross-reacted with 5 strains of Candida albicans and also cross-reacted with Candida tropicalis and Candida stellatoidia and S. cerevisae but did not cross-react with T. glabrata
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to isolate surface components and determine the chemical structures that are responsible for the immunological differences. This Mab would facilitate the development of serological assays
Curator ID	AA + AS
Date of Curation	14-06-2010
References	PMC259333

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PolysacDB ID	1333
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans 3153A (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Cell wall extract of wild-type Candida albicans 3153A
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 17e4
Antibody Type Class	N/A
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This antibody cross-reacted with strains 4B, 4C, 24, 37, 53, and 22 but did not cross-react with 4A, 16, 28, 47, and 62
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab agglutinated Candida albicans cells
Curator ID	AA + AS
Date of Curation	16-06-2010
References	PMC281022

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PolysacDB ID	1334
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans 3153A (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Cell wall extract of wild-type Candida albicans 3153A
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab C6
Antibody Type Class	N/A
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This antibody cross-reacted with strain 62, B, 4C, 24, 37, 53, and 22 but did not react with 4A, 16, 28 and 47 strains
Proposed Epitope	The epitope is supposed to contain high concentrations of glucose and mannose and is present in the acid-labile oligomannosaccharides.
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	18-06-2010
References	PMC281022

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PolysacDB ID	1335
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans 3153A (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Cell wall extract of wild-type Candida albicans 3153A
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab B6
Antibody Type Class	N/A
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross Reactivity	This antibody cross-reacted with strain 62, B, 4C, 24, 37, 53, and 22 but did not react with 4A, 16, 28 and 47 strains
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	18-06-2010
References	PMC281022

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PolysacDB ID	1344
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Live C. albicans blastoconidia
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 5B2
Antibody Type Class	IgM
Assay System	Immunofluorescence and precipitin reactions
Cross Reactivity	This Mab reacted with C. albicans, C. tropic alis, C. glabr-ata, and C. Giiillierrnondii
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab had the ability to agglutinate Candida species
Curator ID	AA + AS
Date of Curation	24-06-2010
References	PMC260140

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PolysacDB ID	1373
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Chlamydomonas reinhardtii strain 21gr (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Chlamydomonas reinhardtii flagellar extract
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	FMG-1
Antibody Type Class	IgG1
Assay System	ELISA, immunoblotting and immunofluorescence
Cross Reactivity	Was specific to Chlamydomonas reinhardtii and did not cross-react with Chlamydomonas moewusi
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	The FMG-1 Monoclonal Antibody Inhibits Flagellar Surface Motility
Curator ID	AA + AS
Date of Curation	01-07-2010
References	PMC2114210

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PolysacDB ID	1374
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Coccidioides immitis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Coccidiodin along with spherulin
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab
Antibody Type Class	IgG1
Assay System	ELISA, immunoblotting and 2d-immunoelectrophoresis
Cross Reactivity	This antibody was specific to Coccidiodes and did not cross-react with histoplasmin and blastocyanin
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb would be useful as a probe for detecting circulating antigen in patients with coccidioidal disease and to assess the effect of passively transferred Mab on the pathogenesis of experimental murine coccidioidomycosis
Curator ID	AA + AS
Date of Curation	02-07-2010
References	PMC313225

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PolysacDB ID	1375
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Coxiela burnetti (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Chloroform-methanol extraction residue of phase I whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab I-1
Antibody Type Class	IgG3
Assay System	ELISA, immunodiffusion and immunoblotting
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb may be useful reagents to monitor the frequency and appearance of specific phase I determinants during phase variation so that the genetic mechanism can be studied. This Mab may also help in the characterization of surface antigenic sites
Curator ID	AA + AS
Date of Curation	02-07-2010
References	PMC263449

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PolysacDB ID	1378
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Oocyst cell wall preparation
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab Cry104
Antibody Type Class	IgG1
Assay System	ELISA and immunoblotting
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	Cry104 gave the tightest cluster of oocysts and the best separation from debris in concentrated environmental water samples
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC95949

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PolysacDB ID	1379
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Supernatant from sonicated Oocysts
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 7B3
Antibody Type Class	N/A
Assay System	Immunoprecipitation and immunofluorescence
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a potential target for passive immunotherapy against cryptosporidiosis
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC258288

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PolysacDB ID	1380
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Supernatant from sonicated Oocysts
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 10C6
Antibody Type Class	N/A
Assay System	Immunoprecipitation and immunofluorescence
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a potential target for passive immunotherapy against cryptosporidiosis
Curator ID	AA + AS
Date of Curation	03-07-2010
References	PMC258288

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PolysacDB ID	1383
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Postexcystation preparation obtained from oocysts
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab TOU
Antibody Type Class	IgG
Assay System	Immunoelectron microscopy and immunoblotting
Cross Reactivity	This Mab cross-reacted with 6 different strains of Cryptosporidium parvum
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab will help in the elucidation of cellular localization of micronemes and its glycoproteinic nature
Curator ID	AA + AS
Date of Curation	04-07-2010
References	PMC257905

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PolysacDB ID	1394
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	C. parvum oocyst extracts
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3D8 2B11
Antibody Type Class	IgA
Assay System	ELISA
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	08-07-2010
References	PMC96563

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PolysacDB ID	1395
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Cryptosporidium parvum (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	C. parvum oocyst extracts
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 3F10 1G3
Antibody Type Class	IgA
Assay System	ELISA
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	08-07-2010
References	PMC96563

Record - 23 of 120 [TOP]

PolysacDB ID	1466
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Helicobacter pylori D273 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab HP15/36
Antibody Type Class	IgM
Assay System	ELISA, Immunoelectron microscopy, immunofluorescence and immunoperoxidase assays
Cross Reactivity	This Mab was specific to Helicobacter pylori and did not cross-react with other Helicobacter species (H. fennelliae and H.mustelae) did not react
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may yield useful information concerning the real surface of the bacterium
Curator ID	AA + AS
Date of Curation	26-07-2010
References	PMC280910

Record - 24 of 120 [TOP]

PolysacDB ID	1467
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Helicobacter pylori D273 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab HP37
Antibody Type Class	IgM
Assay System	ELISA, Immunoelectron microscopy, immunofluorescence and immunoperoxidase assays
Cross Reactivity	This Mab was specific to Helicobacter pylori and did not cross-react with other Helicobacter species (H. fennelliae and H.mustelae) did not react
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may yield useful information concerning the real surface of the bacterium
Curator ID	AA + AS
Date of Curation	26-07-2010
References	PMC280910

Record - 25 of 120 [TOP]

PolysacDB ID	1468
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe Name	Helicobacter pylori D273 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab HP16
Antibody Type Class	IgM
Assay System	ELISA, Immunoelectron microscopy, immunofluorescence and immunoperoxidase assays
Cross Reactivity	This Mab was specific to Helicobacter pylori and did not cross-react with other Helicobacter species (H. fennelliae and H.mustelae) did not react
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab may yield useful information concerning the real surface of the bacterium
Curator ID	AA + AS
Date of Curation	27-07-2010
References	PMC280910

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020