PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID2435
Carbohydrate NameTrisaccharide part of Streptococcus pneumoniae type 3 polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameStreptococcus pneumoniae Type 3   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugates
Carrier NameDiphtheria toxin mutant (CRM197)
Conjugate MethodThe saccharide fragments described above were conjugated to CRM197 using diethyl squarate as linker. Elongation of the saccharides with diethyl squarate was performed in ethanol/0.1M sodium phosphate (pH 6.9). The reaction products were purified by solid-phase extraction and coupled to protein in 0.1m sodium borate buffer at pH 9.5
AntibodiesAntisera
Antibody Type ClassN/A
Assay SystemEnzyme-linked immunosorbent assay and in-vitro phagocytosis assay
Cross ReactivityN/A
Proposed Epitopeβ-d-Glcp-(1-->3)-β-d-GlcpA-(1-->4)-β-d-Glcp
IEDB EpitopeN/A
Proposed UtilityThis antisera provided full protection against challenge with pneumococcal type 3 bacteria
Curator IDAA + AS
Date of Curation13-05-2011
ReferencesPMC308892

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