PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID2461
Carbohydrate NameSynthetic disaccharide abequose(1-->3)-α-rhamnose representative of Salmonella O-antigen 8   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
Microbe NameSalmonella spp.   (NCBI Taxonomy)   (Drugpedia)
Basic StructureSynthetic disaccharide abequose1-->3α-rhamnose
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureGlycoconjugate
Carrier NameBovine serum albumin
Conjugate MethodN/A
AntibodiesAntisera
Antibody Type ClassN/A
Assay SystemImmunofluorescence and co-agglutination (COA)
Cross ReactivityThe antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci
Curator IDAA + AS
Date of Curation23-05-2011
References373381

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