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| PolysacDB ID | 1096 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 7F5 |
| Antibody Type Class | IgG2a |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 28-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1097 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 5B8 |
| Antibody Type Class | IgG2b |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 28-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1098 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 7E6 |
| Antibody Type Class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 28-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1099 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 5G4 |
| Antibody Type Class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 29-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1100 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 4f1 |
| Antibody Type Class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 29-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1101 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 4B8 |
| Antibody Type Class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 29-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1102 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 7E10 |
| Antibody Type Class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 25 serotype 4 isolates,10 serotype 7 isolates, recognized to a lesser extent the isolate belonging to serotype 8 and several serotype 1, 3, and nontypeable isolates |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 29-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1106 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 3E12 |
| Antibody Type Class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 31-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1107 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polysaccharide structure has a branched tetrasaccharide repeating unit {-->3)-β-d-Galp-(1-->4)-[β-d-Glcp-(1-->3)]-β-d- GalpNAc-(1-->4)-α-l-Rhap-(1-}n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7. |
| BCSDB Structure | 115743 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 7C3 |
| Antibody Type Class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross Reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. were reactive with 26 serotype 4 isolates,11 serotype 7 isolates, and 1 each of the serotype 1 and non-typeable isolates tested |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 31-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1110 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 5 FMV81-750 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 108568 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalinized whole cell antigen (WCA) |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 4.4C6.F7 |
| Antibody Type Class | IgG2 |
| Assay System | ELISA, immunoblotting |
| Cross Reactivity | This antibody specifically reacted with Serotype5 only |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for serotyping analysis in actinobacillus strains |
| Curator ID | AA + AS |
| Date of Curation | 01-02-2010 |
| References | 9249182 |
| PolysacDB ID | 1111 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 5 strain 91-178-2005 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 108568 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalinized whole cell antigen (WCA) |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 6.1E7.E3 |
| Antibody Type Class | IgM |
| Assay System | ELISA, immunoblotting |
| Cross Reactivity | This antibody specifically reacted with Serotype5 only |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for serotyping analysis in actinobacillus strains |
| Curator ID | AA + AS |
| Date of Curation | 01-02-2010 |
| References | 9249182 |
| PolysacDB ID | 1113 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 5a (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 108568 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 210-Fll |
| Antibody Type Class | IgG3 |
| Assay System | Elisa inhibition assay |
| Cross Reactivity | This antibody reacted only with the reference strains of Actinobacillus serotypes 5a and 5b. |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used for identification of serotype5 cells |
| Curator ID | AA + AS |
| Date of Curation | 02-02-2010 |
| References | PMC1189361 |
| PolysacDB ID | 1114 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 5b (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 108568 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Antisera |
| Antibody Type Class | N/A |
| Assay System | Elisa inhibition assay |
| Cross Reactivity | This antibody reacted only with the reference strains of Actinobacillus serotypes 5 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used for identification of serotype5 cells |
| Curator ID | AA + AS |
| Date of Curation | 02-02-2010 |
| References | PMC1189361 |
| PolysacDB ID | 1115 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 3E10 |
| Antibody Type Class | IgG2b |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross Reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 03-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1116 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 4B7 |
| Antibody Type Class | IgG2a |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross Reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 03-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1117 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 9H5 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross Reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 03-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1118 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 11C3 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross Reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 04-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1119 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 3B10 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross Reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 04-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1120 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 9Hl |
| Antibody Type Class | IgM |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross Reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 05-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1121 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 5D6 |
| Antibody Type Class | IgM |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross Reactivity | This antibody cross-reacted with reference strains of serotypes 1 and 11 |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 06-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1122 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 1 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with serotypes 9 and 11 |
| Proposed Epitope | The epitope was deduced to contain the terminal nonreducing -D-Glc NAc residues |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera not only helped in serotyping analysis but also helped in deducing the epitopes recognized |
| Curator ID | AA + AS |
| Date of Curation | 06-02-2010 |
| References | PMC206369 |
| PolysacDB ID | 1123 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 9 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The structure consists of trisaccharide backbone randomly substituted by N-acetyl-β-D-glucosamine end groups (to the extent of 25%) |
| BCSDB Structure | 131785 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Formalin-fixed A. pleuropneumoniae serotype 9 cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Polysera |
| Antibody Type Class | N/A |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with serotypes 1 and 12. also cross-reacted with deaminated Serotype1 O-PS |
| Proposed Epitope | The epitope was deduced to contain the terminal nonreducing -D-Glc NAc residues. A second epitope was said to be directed to linear regions of the backbone polymer |
| IEDB Epitope | N/A |
| Proposed Utility | This antisera not only helped in serotyping analysis but also helped in deducing the epitopes recognized |
| Curator ID | AA + AS |
| Date of Curation | 06-02-2010 |
| References | PMC206369 |
| PolysacDB ID | 1136 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 5 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 108568 |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | 5Mab-1 |
| Antibody Type Class | IgM |
| Assay System | ELISA inhibition tests |
| Cross Reactivity | This Mab was specific to serotype 5 strains |
| Proposed Epitope | The epitope was proposed to consist of of β-1,6-linked D-galactose residues |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used for specially distinguishing serotype 5 strains |
| Curator ID | AA + AS |
| Date of Curation | 12-02-2010 |
| References | 1384404 |
| PolysacDB ID | 1138 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Actinobacillus pleuropneumoniae serotype 1 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed Function | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature | Whole cells |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | LMab-5 |
| Antibody Type Class | IgM |
| Assay System | ELISA inhibition tests |
| Cross Reactivity | IMAb-5 reacted with serotype 1, 9 and 11 strains at the same degree and its epitope was found to be located on O-polysaccharide of serotype 1, 9 or 11 lipopolysaccharide (LPS) |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be useful for serotyping studies |
| Curator ID | AA + AS |
| Date of Curation | 13-02-2010 |
| References | 1382625 |
| PolysacDB ID | 1225 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Brucella abortus 2308 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Brucella O-PS structure has been defined as homopolymers of 4,6-dideoxy-4-formamido-α-D-mannopyranose residues |
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Irradiated cells of B. abortus smooth strain 2308 |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | Mab 34 |
| Antibody Type Class | N/A |
| Assay System | ELISA, invivo protection studies |
| Cross Reactivity | N/A |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab confers protection in mice against experimental brucellosis |
| Curator ID | AA + AS |
| Date of Curation | 23-03-2010 |
| References | PMC260994 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |