PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
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PolysacDB ID1345
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans NIH A-207   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureWhole cells
Carrier NameNil
Conjugate MethodNil
AntibodiesPolyclonal Antisera
Antibody Type ClassN/A
Assay SystemOuchterlony double immunodiffusion
Cross ReactivityThe antisera did not cross-react with C. albicans NIH B-792 but cross-reracted with Hormodendrum strains, H. pedrosoi IFO 6071, H. compactum IFO 6726, and H. dermatitidis IFO 6421
Proposed EpitopeThe epitope was proposed to consist of α-D-mannopyranosyl residues occupying the nonreducing terminals of the branching moieties
IEDB Epitope76848
Proposed UtilityThis antisera was used to study the structural similarities between Candida mannans and Hormemendrum galactomannans
Curator IDAA + AS
Date of Curation24-06-2010
ReferencesPMC260140

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PolysacDB ID1346
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans NIH B-792   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB Structure104624
Proposed FunctionN/A
Antigenic NatureWhole cells
Carrier NameNil
Conjugate MethodNil
AntibodiesPolyclonal Antisera
Antibody Type ClassN/A
Assay SystemOuchterlony double immunodiffusion
Cross ReactivityThe antisera cross-reacted with C. albicans NIH A-207 and Hormodendrum strains, H. pedrosoi IFO 6071, H. compactum IFO 6726, and H. dermatitidis IFO 6421
Proposed EpitopeThe epitope was proposed to consist of α-D-mannopyranosyl residues occupying the nonreducing terminals of the branching moieties
IEDB Epitope76848
Proposed UtilityThis antisera was used to study the structural similarities between Candida mannans and Hormemendrum galactomannans
Curator IDAA + AS
Date of Curation24-06-2010
ReferencesPMC550701

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PolysacDB ID1347
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans 526B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureCell walls of C. albicans 526B
Carrier NameNil
Conjugate MethodNil
AntibodiesMab AC3
Antibody Type ClassIgM
Assay SystemIndirect Enzyme Immunoassay
Cross ReactivityThis Mab cross-reacted with Candida albicans 20A and Candida tropicalis
Proposed EpitopeN/A
IEDB Epitope76848
Proposed UtilityThis Mab will help in the serotyping of Candida species
Curator IDAA + AS
Date of Curation24-06-2010
ReferencesPMC269031

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PolysacDB ID1348
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans 526B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureCell walls of C. albicans 526B
Carrier NameNil
Conjugate MethodNil
AntibodiesMab DC5
Antibody Type ClassIgG
Assay SystemIndirect Enzyme Immunoassay
Cross ReactivityThis Mab cross-reacted with Candida albicans 20A and Candida tropicalis
Proposed EpitopeN/A
IEDB Epitope76848
Proposed UtilityThis Mab will help in the serotyping of Candida species
Curator IDAA + AS
Date of Curation24-06-2010
ReferencesPMC269031

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PolysacDB ID1349
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans 20A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureCell walls of C. albicans 20A
Carrier NameNil
Conjugate MethodNil
AntibodiesMab BC4
Antibody Type ClassIgM
Assay SystemIndirect Enzyme Immunoassay
Cross ReactivityThis Mab did not cross-react Candida albicans 526B
Proposed EpitopeN/A
IEDB Epitope76848
Proposed UtilityThis Mab will help in the serotyping of Candida species
Curator IDAA + AS
Date of Curation25-06-2010
ReferencesPMC269031

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PolysacDB ID1350
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans 20A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureCell walls of C. albicans 20A
Carrier NameNil
Conjugate MethodNil
AntibodiesMab CB6
Antibody Type ClassIgM
Assay SystemIndirect Enzyme Immunoassay, immunofluorescence
Cross ReactivityThis Mab did not cross-react Candida albicans 526B
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab will help in the serotyping of Candida species
Curator IDAA + AS
Date of Curation25-06-2010
ReferencesPMC269031

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PolysacDB ID1351
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureHeat-killed cell suspension of C. albicans M1012(serotype A)
Carrier NameNil
Conjugate MethodNil
AntibodiesMab CA4-2
Antibody Type ClassIgM
Assay SystemSlide agglutination tests, immunofluorescence
Cross ReactivityThis Mab cross-reacted with C. tropicalis, and T. glabrata strains
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had agglutinating properties and may be useful also as reagents for revealing the immunochemical structures of specific determinants of C. albiccanis cell wall mannans
Curator IDAA + AS
Date of Curation26-06-2010
ReferencesPMC268742

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PolysacDB ID1352
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
Microbe NameCandida albicans serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed FunctionN/A
Antigenic NatureHeat-killed cell suspension of C. albicans M1012(serotype A)
Carrier NameNil
Conjugate MethodNil
AntibodiesMab CA5-4
Antibody Type ClassIgM
Assay SystemSlide agglutination tests, immunofluorescence
Cross ReactivityThis Mab cross-reacted with C. albicans serotype B, and C. tropicalis strains
Proposed EpitopeN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had agglutinating properties and may be useful also as reagents for revealing the immunochemical structures of specific determinants of C. albicans cell wall mannans
Curator IDAA + AS
Date of Curation26-06-2010
ReferencesPMC268742

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