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| PolysacDB ID | 1003 |
| Carbohydrate Name | Glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Mycobacterium tuberculosis (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | N/A |
| Proposed Function | Activates leukocytes, stimulating their phagocytic, cytotoxic, and antimicrobial activities, including the production of reactive oxygen and nitrogen intermediates |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Pseudomonas aeruginosa exoprotein A (rEPA) |
| Conjugate Method | The polysaccharide was activated with CDAP. A 60-ml volume of CDAP (100 mg/ml of acetonitrile) was added to a solution of polysaccharide (2 ml, 10 mg of polysaccharide per ml of PFS) at room temperature. The pH was maintained at 5.8 to 6.0 for 30 s, and 60 ml of 0.2 M TEA was added to a pH of 7.0. The reaction was carried out for 2 min, and an equal volume of 0.8 M adipic acid dihydrazide [ADH] in 0.5 M NaHCO3 was added. This reaction was carried out for 2 h, and the pH was maintained at 8.0 to 8.5 with 0.1 N NaOH. The reaction mixture was dialyzed against PFS and passed through a column (3 by 46 cm) of P-10 in PFW. The void volume fractions were pooled, freeze-dried. ADH-derivatized polysaccharide (10 mg) was dissolved in PFS (2 ml). An equal weight of protein was added, and the pH was maintained at 5.1 to 5.5 with 0.1 M HCl. The reaction mixture was put on ice, EDAC was added to a final concentration of 0.05 M, and the pH was maintained at 5.1 to 5.5 for 4 h in 0.1 M HCl. The reaction mixtures were dialyzed against 0.2 M NaCl for 2 days with three changes of outer fluid and were passed through a column (1.5 by 90 cm) of Sepharose CL-6B in 0.2 M NaCl. The void volume fractions were stored at 3 to 88°C |
| Antibodies | Mab 24c5 |
| Antibody Type Class | IgG |
| Assay System | ELISA, Immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with M. kansasii, BCG Pasteur, M. smegmatis, M. phlei, M. fortuitum, and M. avium |
| Proposed Epitope | N\A |
| IEDB Epitope | N/A |
| Proposed Utility | The antibody was used to study M. tuberculosis Glucan expression in vitro and in vivo |
| Curator ID | AA + AS |
| Date of Curation | 01-01-2010 |
| References | PMC127896 |
| PolysacDB ID | 1014 |
| Carbohydrate Name | Glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Candida albicans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | 125281 |
| Proposed Function | Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid CRM 197 |
| Conjugate Method | A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices |
| Antibodies | Mab 2G8 |
| Antibody Type Class | IgG2 |
| Assay System | Flow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay |
| Cross Reactivity | This antibody binds both C. neoformans and Candida albicans |
| Proposed Epitope | N\A |
| IEDB Epitope | 76671 |
| Proposed Utility | MAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo. |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC2168274 |
| PolysacDB ID | 1042 |
| Carbohydrate Name | Glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Neurospora crassa (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | N/A |
| Proposed Function | Activates leukocytes, stimulating their phagocytic, cytotoxic, and antimicrobial activities, including the production of reactive oxygen and nitrogen intermediates |
| Antigenic Nature | Homogenous suspension of hyphal walls. |
| Carrier Name | Nil |
| Conjugate Method | Nil |
| Antibodies | S4D1 |
| Antibody Type Class | IgM |
| Assay System | ELISA, immunofluorescence |
| Cross Reactivity | This antibody was cross-reactive with A.nidulans, Schiz.commune. |
| Proposed Epitope | 1,3-α-glucan |
| IEDB Epitope | N/A |
| Proposed Utility | Screening of fungi for presence of 1,3-α-glucan in hyphae cell wall. |
| Curator ID | AA + AS |
| Date of Curation | 13-01-2010 |
| References | 9245814 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |