PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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PolysacDB ID	1004
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Haemophilus influenzae type B (NCBI Taxonomy) (Drugpedia)
Basic Structure	A relatively simple antigen consisting of repeating units of 3-β-D ribose-(1-->1)-D-ribitol-5-phosphate
BCSDB Structure	23423
Proposed Function	An important virulence determinant
Antigenic Nature	Glycoconjugates
Carrier Name	Neisseria meningitidis group B outer membrane protein complex (Hib PS-OMP)
Conjugate Method	The strategy uses a oligopeptide spacer molecule whose parts are derived from both modified Haemophilus influenzae capsular polysaccharide and Neisseria meningitides group B outer membrane complex
Antibodies	Polysera
Antibody Type Class	IgG1 and IgG2
Assay System	N\A
Cross Reactivity	N/A
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	These antibodies exert their protective effect by initiating complement-mediated activities including opsonization and bacterial lysis
Curator ID	AA + AS
Date of Curation	01-01-2010
References	PMC296547

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PolysacDB ID	1005
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Haemophilus influenzae type B (NCBI Taxonomy) (Drugpedia)
Basic Structure	A relatively simple antigen consisting of repeating units of 3-β-D ribose-(1-->1)-D-ribitol-5-phosphate
BCSDB Structure	23423
Proposed Function	An important virulence determinant
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	Protein solutions (25 mg/ml) and Adipic acid dihydrazide [ADH] (3.45 rag/rag protein) were reacted with three different concentrations of EDAC (0 1, 0.3, and 0.6 rag/rag protein) The pH of the reaction mixture was maintained at 4.7 ± 0.2 with 0.1 N HC! The reaction proceeded at room temperature for 3 h and the reaction mixtures were dialyzed at 3-8°C with two changes/d against 6 liter of 0 2 M NaCI. The albumin and polysaccharide derivatives were then dialyzed against two 6-liter changes of deionized water and freeze-dried. The polysaccharide was activated with CNBr. Briefly, a solution of polysaccharide (5.0 mg/ml), equilibrated at 4°C, was rapidly brought to pH 10.5 with 0.1 N NaOH. 100 mg/ml CNBr was added to a final concentration of 0.4 mg/mg polysaccharide, and the pH maintained at 10.5 for 6 rain. Then the reaction mixture was brought to pH 8 5 with 0.5 M NaHCO3, and the CNBr-actlvated polysaccharide added to an equal weight of ADH-protein. The reaction mixture was tumbled gently overnight at 3-8°C and then centrifuged at 16,000 g, 4°C for 20 ram. The supernatant was passed through a CL-4B Sepharose column, 1 5 × 90 cm, that was equihbrated with 0 2 M ammonium acetate. The void-volume fractions were pooled, dialyzed against 0.01 M phosphate-buffered 0.145 M NaCI, pH 7.0, at 3-8°C, and passed through a 045-nm membrane and stored at 3-8°C
Antibodies	Polysera
Antibody Type Class	IgG and IgA
Assay System	Radioimmunoassay
Cross Reactivity	N/A
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	These antibodies exert their protective effect by initiating complement-mediated activities including opsonization and bacterial lysis
Curator ID	AA + AS
Date of Curation	02-01-2010
References	PMC2185954

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PolysacDB ID	1012
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Streptococcus Group B (NCBI Taxonomy) (Drugpedia)
Basic Structure	A basic backbone of the following residues: -3-β-D-Galp-(1-->4) -β-D-Glcp[branched to 1-->4-D-Glp-α-D-NeuNACp]-(1-->6)-β-D-GlcNAcp-1-
BCSDB Structure	6237
Proposed Function	Invades the blood stream and multiply. This property of invasiveness is related to the anti-phagocytic properties conferred by its Capsular polysaccharide
Antigenic Nature	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugate Method	Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C in a pH stat. Adipic acid dihydrazide [AH] was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. After tumbling for 18 h at 3 to 8°C, the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The polysaccharide-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and tetanus toxoid [TT} per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M, and the pH was maintained at 5.6 with 0.1 N NaOH for 3 h at room temperature. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column (5 by 95 cm) equilibrated in 0.2 M NaCl. The void volume fractions were stored in 0.01% thimerosal at 3 to 8°C
Antibodies	Polysera
Antibody Type Class	Mainly IgG, particularly IgG1 and IgG3
Assay System	Double immunodiffusion, capillary precipitation, ELISA
Cross Reactivity	N/A
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	Antibodies elicited by the conjugates had in vitroopsonic activities proposed to be a correlate of protective immunity
Curator ID	AA + AS
Date of Curation	04-01-2010
References	PMC258520

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PolysacDB ID	1016
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 21D2
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody cross-reacted with Serotype A, D and GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1017
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 14A12
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1018
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 11E2
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1019
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 7B13
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	05-01-2010
References	PMC2118886

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PolysacDB ID	1020
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 12G5
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

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PolysacDB ID	1021
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 20C5
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

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PolysacDB ID	1022
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 20B5
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	06-01-2010
References	PMC2118886

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PolysacDB ID	1023
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 4H3
Antibody Type Class	IgG3
Assay System	ELISA
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	07-01-2010
References	PMC2118886

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PolysacDB ID	1024
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure	113471
Proposed Function	The capsular polysaccharide is an important virulence factor
Antigenic Nature	Cryptococci cells
Carrier Name	N/A
Conjugate Method	N/A
Antibodies	Mab 15C6
Antibody Type Class	IgA
Assay System	Immunofluorescence
Cross Reactivity	This antibody was specific to D serotype and cross-reacted with GH strain
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	This antibody was shown to bind to cryptococcal capsule
Curator ID	AA + AS
Date of Curation	07-01-2010
References	PMC2118886

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PolysacDB ID	1070
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Neisseria meningitidis Serogroup B (NCBI Taxonomy) (Drugpedia)
Basic Structure	The capsular polysaccharide, also known as K-antigen consists of repeating units of following residues : -8)α-Neup5Ac(2- [a homopolymer of α(2-->8) sialic acid residues]
BCSDB Structure	139710
Proposed Function	N/A
Antigenic Nature	Live group B type 15 meningococci cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 2-2-B
Antibody Type Class	IgM
Assay System	ELISA and Radioimmunoassay
Cross Reactivity	This antibody cross-reacted with E. coli strain K1 Capsular polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab provided protection against the E. coli K-1 strain
Curator ID	AA + AS
Date of Curation	21-01-2010
References	PMC261141

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PolysacDB ID	1109
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 1 FMV87-682 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalinized whole cell antigen (WCA)
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab F11-7F
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting
Cross Reactivity	This Mab did not recognize other bacterial species, such as A. lignieresii, A. suis, A. rossii, Haemophilus spp., H. parasuis, P. multocida, P. suis, B. bronchiseptica, and E. coli
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab was proposed to stabilize the capsule.This MAb could be useful for serotyping analysis in actinobacillus strains
Curator ID	AA + AS
Date of Curation	01-02-2010
References	9249182

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PolysacDB ID	1112
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Formalinized whole cell antigen (WCA)
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 1.5C5F4
Antibody Type Class	IgM
Assay System	ELISA, immunoblotting
Cross Reactivity	This antibody specifically reacted with Serotype1 only
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This MAb could be useful for serotyping analysis in actinobacillus strains
Curator ID	AA + AS
Date of Curation	02-02-2010
References	9249182

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PolysacDB ID	1124
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 1 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	N/A
Assay System	ELISA
Cross Reactivity	This antibody was specific to serotype 1 only
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	07-02-2010
References	PMC206369

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PolysacDB ID	1137
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Actinobacillus pleuropneumoniae serotype 1 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	LMab-1
Antibody Type Class	IgM
Assay System	ELISA inhibition tests
Cross Reactivity	LMAb-1 reacted to only a serotype 1, strain 4074
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab can be useful for serotyping studies
Curator ID	AA + AS
Date of Curation	13-02-2010
References	1382625

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PolysacDB ID	1142
Carbohydrate Name	Capsular Polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Bacteroides fragilis NCTC 9343 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature	Whole cells of B. fragilis NCTC 9343
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab BF4
Antibody Type Class	IgM
Assay System	ELISA, Enzyme Immunoassay and immunoblotting
Cross Reactivity	MAb Bf4 did not cross react with the sixteen Bacteroides species and strains tested
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	14-02-2010
References	3309142

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PolysacDB ID	1157
Carbohydrate Name	Capsular Polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Bacteroides fragilis strain 638R (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	Capsular polysaccharide of B. fragilis may represent a virulence factor
Antigenic Nature	Whole cells of B. fragilis strain 638R
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	N/A
Assay System	Immunodiffusion
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera helped in the discovery of 3 different types of surface polysaccharides on the capsule
Curator ID	AA + AS
Date of Curation	21-02-2010
References	PMC98163

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PolysacDB ID	1463
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Enterococcus faecalis (NCBI Taxonomy) (Drugpedia)
Basic Structure	Glycerol teichoic acid-like molecules with a carbohydrate backbone structure of 6-α-D-glucose- 1-->2 glycerol-3-PO4 with substitution on carbon 2 of the glucose with an α-2-1-D-glucose residue
BCSDB Structure	2446
Proposed Function	These polysaccharides are major virulence factors
Antigenic Nature	Nil
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Healthy human sera
Antibody Type Class	IgG and IgM
Assay System	ELISA
Cross Reactivity	The antisera bound to all 4 serotypes of E. fecalis CPS-A to CPS-D
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	These antibodies promote neutrophil mediated killing
Curator ID	AA + AS
Date of Curation	24-07-2010
References	PMC1182185

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PolysacDB ID	1464
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Enterococcus faecalis 12030 (NCBI Taxonomy) (Drugpedia)
Basic Structure	Glycerol teichoic acid-like molecules with a carbohydrate backbone structure of 6-α-D-glucose- 1-->2 glycerol-3-PO4 with substitution on carbon 2 of the glucose with an α-2-1-D-glucose residue
BCSDB Structure	2446
Proposed Function	These polysaccharides are major virulence factors
Antigenic Nature	Gentamicin-killed Enterococcus faecalis strains
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	N/A
Assay System	Opsonophagocytic assay, immunoelectron microscopy
Cross Reactivity	This antisera reacted with the homologous strain (12030) and mediated opsonic killing of 33% of all strains tested. In addition, this serum killed two (28%) of seven vancomycin-resistant Enterococcus faecium strains
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera was opsonophagcytic
Curator ID	AA + AS
Date of Curation	24-07-2010
References	10024563

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PolysacDB ID	1465
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Enterococcus faecalis 12030 (NCBI Taxonomy) (Drugpedia)
Basic Structure	Glycerol teichoic acid-like molecules with a carbohydrate backbone structure of 6-α-D-glucose- 1-->2 glycerol-3-PO4 with substitution on carbon 2 of the glucose with an α-2-1-D-glucose residue
BCSDB Structure	2446
Proposed Function	These polysaccharides are major virulence factors
Antigenic Nature	Capsular polysaccharide
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	IgM
Assay System	ELISA and opsonophagocytic assay
Cross Reactivity	The antisera cross-reacted with Enterococcus faecalis OG1RF and against two serologically related, vancomycin-resistant Enterococcus faecium clinical isolates
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	These antibodies may be useful for prophylaxis and treatment of enterococcal infections
Curator ID	AA + AS
Date of Curation	25-07-2010
References	15184433

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PolysacDB ID	1494
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans Serotype A [strain 24064] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues
BCSDB Structure	N/A
Proposed Function	C. neoformans has a large polysaccharide capsule that is a determinant of virulence. The capsular polysaccharide causes immunologic paralysis and inhibits phagocytosis by macrophages. Soluble C. neoformans capsular polysaccharide enhances human immunodeficiency virus [HIV] infection in cultured cells, suggesting a new pathogenic role in AIDS
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	IgG [predominantly IgG1]
Assay System	ELISA
Cross Reactivity	This antibody cross-reacted with Serotype D strains
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	03-08-2010
References	1583327

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PolysacDB ID	1495
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans Serotype A [strain 24064] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues
BCSDB Structure	N/A
Proposed Function	C. neoformans has a large polysaccharide capsule that is a determinant of virulence. The capsular polysaccharide causes immunologic paralysis and inhibits phagocytosis by macrophages. Soluble C. neoformans capsular polysaccharide enhances human immunodeficiency virus [HIV] infection in cultured cells, suggesting a new pathogenic role in AIDS
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Polysera
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody cross-reacted with Serotype D strains
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	03-08-2010
References	1583327

Record - 25 of 162 [TOP]

PolysacDB ID	1497
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans Serotype A [strain 24064] (NCBI Taxonomy) (Drugpedia)
Basic Structure	The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues
BCSDB Structure	N/A
Proposed Function	The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues
Antigenic Nature	Whole cells
Carrier Name	Nil
Conjugate Method	Nil
Antibodies	Mab 5E9
Antibody Type Class	IgM
Assay System	ELISA
Cross Reactivity	This antibody reacted with 2 serotype A strains 24064 and 371
Proposed Epitope	Mab 5E9 bound to de-O-acetylated GXM of strain 371 but not of strain 24064. This shows the probable epitope to be variable among strains and to be acetylation independent
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	04-08-2010
References	1583327

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020