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| PolysacDB ID | 1025 |
| Carbohydrate Name | Capsular polysaccharide A (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | N/A |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 439 |
| Antibody Type Class | IgG1 |
| Assay System | Ouchterlony double immunodiffusion, Phagocytosis assays |
| Cross Reactivity | This antibody cross-reacted with all the 4 serotypes- A, B, C and D |
| Proposed Epitope | The reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 07-01-2010 |
| References | PMC260621 |
| PolysacDB ID | 1026 |
| Carbohydrate Name | Capsular polysaccharide A (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | N/A |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 1255 |
| Antibody Type Class | IgG1 |
| Assay System | Ouchterlony double immunodiffusion, Phagocytosis assays |
| Cross Reactivity | This antibody cross-reacted with 3 serotypes- A, B and D |
| Proposed Epitope | The reactivity of this antibody required an intact carboxyl group and also required deacetylation of the parent polysaccharide |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC260621 |
| PolysacDB ID | 1027 |
| Carbohydrate Name | Capsular polysaccharide D (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | N/A |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 302 |
| Antibody Type Class | IgG1 |
| Assay System | Ouchterlony double immunodiffusion, Phagocytosis assays |
| Cross Reactivity | This antibody cross-reacted with 2 serotypes- A and D |
| Proposed Epitope | The reactivity of this antibody required acetylation of the parent polysaccharide. Hence proposed epitopes may be O-acetyl groups |
| IEDB Epitope | N/A |
| Proposed Utility | MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity |
| Curator ID | AA + AS |
| Date of Curation | 08-01-2010 |
| References | PMC260621 |
| PolysacDB ID | 2601 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 130477 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 339 |
| Antibody Type Class | N/A |
| Assay System | ELISA and Immunodiffusion |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes |
| Curator ID | AA + AS |
| Date of Curation | 15-07-2011 |
| References | 2476401 |
| PolysacDB ID | 2602 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype D (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 136943 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 386 |
| Antibody Type Class | N/A |
| Assay System | ELISA and Immunodiffusion |
| Cross Reactivity | This antibody has a highly variable cross-reactivity. It cross reacts with most strains of Serotype A and D. It cross reacts with only one strain of serotype B and 2 strains of serotype C. does not show a reaction with ELISA |
| Proposed Epitope | Since Mab 386 does not show any positive results with ELISA, it is suggested that binding of polysaccharides to plates coated with poly-L-lysine altered the carboxyl residues that may form part of an epitope recognized by the antibody |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes |
| Curator ID | AA + AS |
| Date of Curation | 15-07-2011 |
| References | 2476401 |
| PolysacDB ID | 2603 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 130477 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 471 |
| Antibody Type Class | N/A |
| Assay System | ELISA and Immunodiffusion |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes. This monoclonal antibody promoted opsonization of Cryptococcus cells |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 2476401 |
| PolysacDB ID | 2604 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype C (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 136942 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 3C2 |
| Antibody Type Class | N/A |
| Assay System | ELISA and Immunodiffusion |
| Cross Reactivity | This antibody cross-reacted with Serotype A,B,C and D serotypes |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | Used to differentiate various strains of all serotypes. This monoclonal antibody promoted opsonization of Cryptococcus cells |
| Curator ID | AA + AS |
| Date of Curation | 16-07-2011 |
| References | 2476401 |
| PolysacDB ID | 2612 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated |
| BCSDB Structure | 116826 |
| Proposed Function | The capsular polysaccharide is an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab 1326 |
| Antibody Type Class | IgG1 |
| Assay System | ELISA |
| Cross Reactivity | This antibody was cross-reactive to serotypes A and D |
| Proposed Epitope | N/A |
| IEDB Epitope | 115576 |
| Proposed Utility | This monoclonal antibody promoted opsonization of Cryptococcus cells |
| Curator ID | AA + AS |
| Date of Curation | 18-07-2011 |
| References | 1583327 |
| PolysacDB ID | 2637 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype B (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 9354 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab F10F5 |
| Antibody Type Class | IgG1 |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotypes A and B |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan |
| Curator ID | AA + AS |
| Date of Curation | 28-07-2011 |
| References | 12965925 |
| PolysacDB ID | 2638 |
| Carbohydrate Name | De-O-acetylated Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | 9353 |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugate Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab F12D2 |
| Antibody Type Class | IgG3 |
| Assay System | ELISA |
| Cross Reactivity | This antibody cross-reacted with Serotypes A, B, C and D |
| Proposed Epitope | Studies on this antibody showed that there are 2 types of epitopes on GXM-O-acetyl dependent and O-acetyl independent. MAb F12D2 also fails to react with xylose deficient GXM shopwing that xylose residues form an important part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan. This antibody showed that the induction of a MAb that is reactive with all serotypes does not inherently require the presence of an O-acetyl group on the immunizing GXM |
| Curator ID | AA + AS |
| Date of Curation | 28-07-2011 |
| References | 12965925 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |