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| PolysacDB ID | 1014 |
| Carbohydrate Name | Glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Candida albicans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | 125281 |
| Proposed Function | Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid CRM 197 |
| Conjugate Method | A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices |
| Antibodies | Mab 2G8 |
| Antibody Type Class | IgG2 |
| Assay System | Flow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay |
| Cross Reactivity | This antibody binds both C. neoformans and Candida albicans |
| Proposed Epitope | N\A |
| IEDB Epitope | 76671 |
| Proposed Utility | MAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo. |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC2168274 |
| PolysacDB ID | 1015 |
| Carbohydrate Name | Beta-glucan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Candida albicans (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length |
| BCSDB Structure | 125281 |
| Proposed Function | Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid CRM 197 |
| Conjugate Method | A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices |
| Antibodies | Polysera |
| Antibody Type Class | IgG |
| Assay System | Invivo protection assays, growth inhibition assays, indirect ELISA |
| Cross Reactivity | N/A |
| Proposed Epitope | N\A |
| IEDB Epitope | 76671 |
| Proposed Utility | The Lam-CRM conjugate is immunogenic and protective against systemic candidiasis in mice. The antibodies induce passive protection in mice against systemic and mucosal candidiasis |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC2212864 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |