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| PolysacDB ID | 1571 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe Name | Leptospira interrogans serovar pomona (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The oligosaccharide contained rhamnose, ribose, glucose, and glucosamine |
| BCSDB Structure | N/A |
| Proposed Function | N/A |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid |
| Conjugate Method | The oligosaccharide was modified as follows : it was dissolved in N-ethylmorpholine-water-12 N HCl (2.5:6.5:1 [vol/vol/vol], pH 8.5) in concentrations of approximately 24 umol in 250ul. 125umol of N-succinimidyl S-acetylmercaptoacetate in 250ul of N,N dimethylacetamide was added to the oligosaccharide. This solution was added to modified diphtheria toxoid |
| Antibodies | Polysera |
| Antibody Type Class | IgG and IgM |
| Assay System | Enzyme Immunoassay, immunoblotting and dot blot |
| Cross Reactivity | This antisera cross-reacted with LPS from some other leptospiral serovars (serovars hardjobovis, ballum, copenhageni, canicola, grippotyphosa, and batavia) and LPS from other bacteria (Salmonella typhimurium and the Re mutant of Shigella flexneri) and did not react with serovars australis, grippotyphosa, ballum, or tarassovi |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This antiserum induced a high CL response in mouse macrophage monolayers |
| Curator ID | AA + AS |
| Date of Curation | 10-09-2010 |
| References | PMC303291 |
| PolysacDB ID | 2639 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | N/A |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid |
| Conjugate Method | 5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid |
| Antibodies | Mab G14 |
| Antibody Type Class | IgM |
| Assay System | ELISA and immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with Serotypes A and D |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb mediated complement deposition on solid phase GXM by ELISA |
| Curator ID | AA + AS |
| Date of Curation | 28-07-2011 |
| References | 15271943 |
| PolysacDB ID | 2640 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | N/A |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid |
| Conjugate Method | 5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid |
| Antibodies | Mab G15 |
| Antibody Type Class | IgM |
| Assay System | ELISA and immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with Serotypes A and D in whole cell ELISA and immunofluorescence. Did not cross-react with Serotype A glucurunoxylomannan alone |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb mediated complement deposition on solid phase GXM by ELISA. Administration of Mab G15 also prolonged the survival of C. neoformans |
| Curator ID | AA + AS |
| Date of Curation | 29-07-2011 |
| References | 15271943 |
| PolysacDB ID | 2641 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe Name | Cryptococcus neoformans Serotype D [strain 24067] (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues |
| BCSDB Structure | N/A |
| Proposed Function | Antiphagocytic ; an important virulence factor |
| Antigenic Nature | Glycoconjugates |
| Carrier Name | Diphtheria toxoid |
| Conjugate Method | 5 mg of GXM (strain 24067) was activated with 5 mg of CDAP (1-cyano-4-dimethylaminopyridinium tetrafluoroborate) and conjugated to Diphtheria toxoid |
| Antibodies | Mab G19 |
| Antibody Type Class | IgM |
| Assay System | ELISA and immunofluorescence |
| Cross Reactivity | This antibody cross-reacted with Serotypes A and D |
| Proposed Epitope | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb mediated complement deposition on solid phase GXM by ELISA |
| Curator ID | AA + AS |
| Date of Curation | 29-07-2011 |
| References | 15271943 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |