PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

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Record - 1 of 29 [TOP]

PolysacDB ID	1028
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure	130362
Proposed Function	The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg]
Antibodies	Mab BD1
Antibody Type Class	IgG1
Assay System	Indirect immunofluorescence
Cross Reactivity	This antibody cross-reacted with 2 serotypes- A and D. BD-1 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus
Proposed Epitope	Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups
IEDB Epitope	N/A
Proposed Utility	MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	08-01-2010
References	PMC259921

Record - 2 of 29 [TOP]

PolysacDB ID	1029
Carbohydrate Name	Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure	130362
Proposed Function	The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed [yield, 17 mg]
Antibodies	Mab BA4
Antibody Type Class	IgM
Assay System	Indirect immunofluorescence
Cross Reactivity	This antibody cross-reacted with 2 serotypes- A and B. BA4 also showed some cross-reactions at a low dilution with B. dermatitidis and H. capsulatum and C. terreus
Proposed Epitope	N\A
IEDB Epitope	N/A
Proposed Utility	MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	08-01-2010
References	PMC259921

Record - 3 of 29 [TOP]

PolysacDB ID	1030
Carbohydrate Name	O-deacetylated Glucurunoxylomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Cryptococcus neoformans Serotype C (NCBI Taxonomy) (Drugpedia)
Basic Structure	The GXM is a high-molecular-weight linear (1-->3)-α-D-mannan substituted with single (1-->2)-β-xylosyl and (1-->2)-β-glucuronosyl residues. 0 acetylation occurs on the C-6 of about half of the mannose residues. The molar ratio of xylose-mannose-glucuronic acid is 2:3:1 for serotype A
BCSDB Structure	73
Proposed Function	The capsular glucuronoxylomannan (GXM) of C. neoformans is anantiphagocytic and tolerogenic polysaccharide and potentiates virulence
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	GXM of C. neoformans serotype A 9759 was activated with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) and was coupled to BSA-AH. GXM (100 mg) was suspended in acetone, and 75 ul of pyridine was added. Tresyl chloride (50 ,ul) was added and stirred for 10 min. The activated GXM was washed with ethanol containing 5 mM HCI, washed with absolute ethanol, and dried in vacuo. Activated GXM (46 mg) was dissolved in 1.5 ml of 0.2 M NaH2PO4 buffer (pH 7.5) (coupling buffer), and BSA-AH was added (25 mg in 0.5 ml of the same buffer). The reaction was stirred overnight at 24°C. The precipitate was removed by centrifugation, and the supernatant was applied to a column (90 by 2.5 cm) of Sepharose CL-6B equilibrated with 0.05 M Tris hydrochloride-0.1 M NaCl buffer (pH 7.6). The flow rate was 35 ml/h. Fractions containing carbohydrate and protein appeared in the void volume. They were pooled, dialyzed, and lyophilized (yield, 33 mg). The conjugate was then rechromatographed (yield, 17 mg)
Antibodies	Mab CD6
Antibody Type Class	IgG1
Assay System	Indirect immunofluorescence
Cross Reactivity	This antibody cross-reacted with all 4 serotypes- A, B, C and D.
Proposed Epitope	Glucuronic acid was proposed to be the important constituent of the epitope. However the epitope also probably contained xylose and was influencedto a minor extent by the presence of O-acetyl groups
IEDB Epitope	N/A
Proposed Utility	The ability to produce a MAb against an epitope shared by many serotypes may have value for the detection of cryptococcal antigens in body fluids. This antibody also has potent opsonic activity
Curator ID	AA + AS
Date of Curation	09-01-2010
References	PMC259921

Record - 4 of 29 [TOP]

PolysacDB ID	1354
Carbohydrate Name	Tetrasaccharide of 3-deoxy-α-d-manno-oct-2-ulopyranosonic acid (Kdo) (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydophila psittaci (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	The allyl glycosides R-OCH2-CH=CH2 were conjugated with cysteamine yielding R-O(CH2)3-S-(CH2)2-NH3+, which were activated with thiophosgene into the isothiocyanate derivatives R O(CH2)3-S-(CH2)2-N=C=S and then conjugated to BSA yielding R-O(CH2)3-S-(CH2)2-NH-CS-NH-BSA, wherein R represents the glycosyl residue
Antibodies	Mab S69-4
Antibody Type Class	IgG1
Assay System	IFT assay, ELISA
Cross Reactivity	This antibody was specific only to C. psittaci
Proposed Epitope	The epitope consists of a branched Kdo(2N/A8)[Kdo(2N/A4)] Kdo(2N/A4)Kdo(2N/A4) tetrasaccharide that is specific to Chlamydia psittaci
IEDB Epitope	76909
Proposed Utility	This Mab may provide insight into the molecular details of the interaction of these antibodies with acidic Kdo containing epitopes. Mab S69-4 can be used in immunohistology to reliably identify C. psittaci at the species level
Curator ID	AA + AS
Date of Curation	26-06-2010
References	16282606

Record - 5 of 29 [TOP]

PolysacDB ID	1363
Carbohydrate Name	α-Kdo-(2->8)-α-Kdo-(2->4)-α-Kdo-(2->6)-β-GlcNAc-(l->6)-α-GlcNAc (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	A-Kdo-(2->8)-a-Kdo-(2->4)-a-Kdo-(2->6)-P-GlcNAc-(l->6)-a-GlcNAc
Carrier Name	Bovine serum albumin
Conjugate Method	Derivatized using a solution of thiophosgene
Antibodies	Mab S40-26
Antibody Type Class	N/A
Assay System	ELISA, immunoblotting and immunofluorescence
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	28-06-2010
References	PMC258430

Record - 6 of 29 [TOP]

PolysacDB ID	1364
Carbohydrate Name	α-Kdo-(2->8)-α-Kdo-(2->4)-α-Kdo-(2->6)-β-GlcNAc-(l->6)-α-GlcNAc (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Derivatized using a solution of thiophosgene
Antibodies	Mab S40-8
Antibody Type Class	N/A
Assay System	ELISA, immunoblotting and immunofluorescence
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	29-06-2010
References	PMC258430

Record - 7 of 29 [TOP]

PolysacDB ID	1366
Carbohydrate Name	Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
Antibodies	Mab S23-24
Antibody Type Class	IgG1
Assay System	Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross Reactivity	This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis and salmonella minnesota R595 but did not cross-react with mycobacteria
Proposed Epitope	This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	29-06-2010
References	PMC256998

Record - 8 of 29 [TOP]

PolysacDB ID	1367
Carbohydrate Name	Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
Antibodies	Mab S25-7
Antibody Type Class	IgM
Assay System	Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross Reactivity	This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis and salmonella minnesota R595 but did not cross-react with mycobacteria
Proposed Epitope	This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	29-06-2010
References	PMC256998

Record - 9 of 29 [TOP]

PolysacDB ID	1368
Carbohydrate Name	Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
Antibodies	Mab S25-27
Antibody Type Class	IgG1
Assay System	Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross Reactivity	This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed Epitope	This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	30-06-2010
References	PMC256998

Record - 10 of 29 [TOP]

PolysacDB ID	1369
Carbohydrate Name	Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
Antibodies	Mab S25-2
Antibody Type Class	IgG1
Assay System	Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross Reactivity	This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed Epitope	This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	30-06-2010
References	PMC256998

Record - 11 of 29 [TOP]

PolysacDB ID	1370
Carbohydrate Name	Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonioc acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
Antibodies	Mab S25-23
Antibody Type Class	IgG1
Assay System	Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross Reactivity	This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed Epitope	This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	30-06-2010
References	PMC256998

Record - 12 of 29 [TOP]

PolysacDB ID	1371
Carbohydrate Name	Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
Antibodies	Mab S25-5
Antibody Type Class	IgG1
Assay System	Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross Reactivity	This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed Epitope	This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	01-07-2010
References	PMC256998

Record - 13 of 29 [TOP]

PolysacDB ID	1372
Carbohydrate Name	Tetrasaccharide 3-deoxy-α-d-manno-2-octulosonic acid (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Chlamydia spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of repeating units of tetrasaccharide of 3-deoxy--D-manno-oct-2 ulopyranosonic acid (Kdo) residues
BCSDB Structure	N/A
Proposed Function	LPS represents one of the major surface antigens of this microorganism
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	Allylglycosides were reacted with cysteamine leading to ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3+, where R represents the respective carbohydrate ligand. These compounds were reacted with acryloyl chloride, resulting in the formation of derivatives of the general formula R-(CH2)3-S-(CH2)2- NH-CO-CH=CH2, which, after copolymerization with acrylamide, yielded polyacrylamide derivatives. Ligands with spacers of the general formula R-(CH2)3-S-(CH2)2-NH3' were activated into isothiocyanate derivatives as follows. Thiophosgene (6 ul) was dissolved in chloroform (3 ml), and a solution of the ligand with spacers (15 mg) in water (3 ml) was added. The reaction was monitored by using thin-layer chromatography on plates of silica gel 60 with chloroformmethanol-water (10/10/3 by volume) as the irrigant and detection with UV light (254-nm wavelength). When the reaction was completed, usually within 3 h of stirring at room temperature, the organic phase was removed and the aqueous layer was extracted three times with 3 ml of chloroform. The aqueous phase was freed from residual chloroform by evaporation under a stream of nitrogen and added to a solution of BSA in 0.3 M sodium chloride containing 0.1 M sodium bicarbonate (1 ml). The reaction mixture was stirred for 48 h at room temperature and then separated by gel permeation chromatography with a 35- by 1.6-cm column of Sephadex G-50 and 10 mM sodium bicarbonate as the eluant. Collected ninhydrin-positive fractions were combined and dialyzed against phosphate-buffered saline (0.15 M, pH 7.2; PBS)
Antibodies	Mab S25-26
Antibody Type Class	IgG1
Assay System	Hemagglutination, Enzyme immunoassay, immunofluorescence, immunoblotting
Cross Reactivity	This Mab cross-reacted with elementary bodies of Chlamydia psittaci and Chlamydia trachomatis but did not cross-react with mycobacteria and and salmonella minnesota R595
Proposed Epitope	This Mab recognizes a trisaccharide epitope αKdo(2-->8) αKdo(2-->4)αKdo
IEDB Epitope	76828
Proposed Utility	This Mab can be used in the epitope characterization of Chlamydial LPS
Curator ID	AA + AS
Date of Curation	01-07-2010
References	PMC256998

Record - 14 of 29 [TOP]

PolysacDB ID	1450
Carbohydrate Name	FVP2 polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Flammulina velutipes (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB Structure	N/A
Proposed Function	FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
Antibodies	Mab B11D
Antibody Type Class	IgM
Assay System	Indirect ELISA, Double Immunodiffusion
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator ID	AA + AS
Date of Curation	14-07-2010
References	19108616

Record - 15 of 29 [TOP]

PolysacDB ID	1451
Carbohydrate Name	FVP2 polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Flammulina velutipes (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB Structure	N/A
Proposed Function	FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
Antibodies	Mab C8G
Antibody Type Class	IgM
Assay System	Indirect ELISA, Double Immunodiffusion
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator ID	AA + AS
Date of Curation	14-07-2010
References	19108616

Record - 16 of 29 [TOP]

PolysacDB ID	1452
Carbohydrate Name	FVP2 polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Flammulina velutipes (NCBI Taxonomy) (Drugpedia)
Basic Structure	Consists of an α-(1-->4)-D-glucan, branching with a single α-D-glucan at the C-6 position to every seven residues along the main chain
BCSDB Structure	N/A
Proposed Function	FVP2 can enhance the livability of primary culture of mouse hepatocytes in vitro and decrease the release of the alanine aminotransferase (ALT) as well as apoptosis of hepatocytes after the carbon tetrachloride (CCl4) intoxication. Furthermore, FVP2 has the capability of suppressing the growth of hepatoma 22 cells and sarcoma 180 cells (S180), extending the survival time of L615 leukemia mice, and promoting the lymphocyte transformation rate, natural killer cell activity, and the interleukin-2 production index in normal and tumor-bearing (S180) mice
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	The FVP2 polysaccharide was conjugated to BSA using 1-cyano-4-dimethyl-aminopyridinium tetrafluoroborate (CDAP) treatment
Antibodies	Mab I6D
Antibody Type Class	IgM
Assay System	Indirect ELISA, Double Immunodiffusion
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab provides a potentially useful tool for further study in the development of a novel and efficient immunoassay to detect FVP2 in biological specimens
Curator ID	AA + AS
Date of Curation	21-07-2010
References	19108616

Record - 17 of 29 [TOP]

PolysacDB ID	1907
Carbohydrate Name	Marine fungal polysaccharide YCP (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB Structure	N/A
Proposed Function	YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies	Mab A7F
Antibody Type Class	IgG3
Assay System	Inhibition ELISA, double immunodiffusion
Cross Reactivity	This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a promising drug candidate
Curator ID	AA + AS
Date of Curation	26-11-2010
References	18991095

Record - 18 of 29 [TOP]

PolysacDB ID	1908
Carbohydrate Name	Marine fungal polysaccharide YCP (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB Structure	N/A
Proposed Function	YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies	Mab L2D
Antibody Type Class	IgM
Assay System	Inhibition ELISA, double immunodiffusion
Cross Reactivity	This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a promising drug candidate
Curator ID	AA + AS
Date of Curation	26-11-2010
References	18991095

Record - 19 of 29 [TOP]

PolysacDB ID	1909
Carbohydrate Name	Marine fungal polysaccharide YCP (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB Structure	N/A
Proposed Function	YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies	Mab L6A
Antibody Type Class	IgG3
Assay System	Inhibition ELISA, double immunodiffusion
Cross Reactivity	This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a promising drug candidate
Curator ID	AA + AS
Date of Curation	26-11-2010
References	18991095

Record - 20 of 29 [TOP]

PolysacDB ID	1910
Carbohydrate Name	Marine fungal polysaccharide YCP (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB Structure	N/A
Proposed Function	YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies	Mab M6C
Antibody Type Class	IgM
Assay System	Inhibition ELISA, double immunodiffusion
Cross Reactivity	This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a promising drug candidate
Curator ID	AA + AS
Date of Curation	26-11-2010
References	18991095

Record - 21 of 29 [TOP]

PolysacDB ID	1911
Carbohydrate Name	Marine fungal polysaccharide YCP (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB Structure	N/A
Proposed Function	YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies	Mab N3F
Antibody Type Class	IgM
Assay System	Inhibition ELISA, double immunodiffusion
Cross Reactivity	This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a promising drug candidate
Curator ID	AA + AS
Date of Curation	27-11-2010
References	18991095

Record - 22 of 29 [TOP]

PolysacDB ID	1912
Carbohydrate Name	Marine fungal polysaccharide YCP (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB Structure	N/A
Proposed Function	YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies	Mab P12B
Antibody Type Class	IgG1
Assay System	Inhibition ELISA, double immunodiffusion
Cross Reactivity	This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a promising drug candidate
Curator ID	AA + AS
Date of Curation	27-11-2010
References	18991095

Record - 23 of 29 [TOP]

PolysacDB ID	1913
Carbohydrate Name	Marine fungal polysaccharide YCP (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Phoma herbarum YS4108 (NCBI Taxonomy) (Drugpedia)
Basic Structure	It has a structure of α-(1-->4)-D-glucan branched with α-(1-->6)-D-linked side chain
BCSDB Structure	N/A
Proposed Function	YCP strongly stimulates both cell-mediated and humoral immunities in in vivo and in vitro conditions. YCP administration could significantly suppress the growth of mouse-transplanted tumor
Antigenic Nature	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugate Method	80 ul of 1-cyano dimethylamino pyridinium tetrafluorate (400 mM in acetonitrile) was added to 2 ml of YCP (4 uM in water). The pH of the reaction mixture was subsequently raised to 9.0 by the addition of 300 mM triethylamine. After 80 ul of BSA (2 mM in 150 mM NaCl) being added, the reaction lasting overnight at C was quenched with 1 ml of 500 mM ethanolamine. The reaction mixture was dialyzed against 10 mM PBS (pH 7.4) and then applied to a Sephacryl S400 column (100 4 1.6 cm) eluted with PBS. The fractions containing the conjugate were pooled, dialyzed against distilled water. The amount of polysaccharide in the conjugate was determined by the sulfuric acid method and the content of protein by s method. Its hapten ratio was estimated as mole of protein per mole of polysaccharide. The conjugate emerged as a single peak in the void volume and entirely separated from free BSA and/or YCP
Antibodies	Mab R10F
Antibody Type Class	IgM
Assay System	Inhibition ELISA, double immunodiffusion
Cross Reactivity	This Mab was highly specific to P. herbarum YCP polysaccharide
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab is a promising drug candidate
Curator ID	AA + AS
Date of Curation	27-11-2010
References	18991095

Record - 24 of 29 [TOP]

PolysacDB ID	2412
Carbohydrate Name	Phosphorylcholine (PC) (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe Name	Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed Function	Antibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial
Antigenic Nature	ASRNKANDYTTEYSASVKGRFIVS [peptide1]
Carrier Name	Bovine serum albumin
Conjugate Method	BSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4°C
Antibodies	Antisera
Antibody Type Class	N/A
Assay System	ELISA, Opsonization and protection assays
Cross Reactivity	N/A
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA
Curator ID	AA + AS
Date of Curation	03-05-2011
References	10992485

Record - 25 of 29 [TOP]

PolysacDB ID	2461
Carbohydrate Name	Synthetic disaccharide abequose(1-->3)-α-rhamnose representative of Salmonella O-antigen 8 (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe Name	Salmonella spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Synthetic disaccharide abequose1-->3α-rhamnose
BCSDB Structure	N/A
Proposed Function	N/A
Antigenic Nature	Glycoconjugate
Carrier Name	Bovine serum albumin
Conjugate Method	N/A
Antibodies	Antisera
Antibody Type Class	N/A
Assay System	Immunofluorescence and co-agglutination (COA)
Cross Reactivity	The antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria
Proposed Epitope	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci
Curator ID	AA + AS
Date of Curation	23-05-2011
References	373381

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020