PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 4

Entry No. - 1   [TOP]
PolysacDB ID1329
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeCandida albicans B311   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesPolyclonal Antisera
Antibody type and classIgM
Assay SystemELISA, immunofluorescence, crossed immunoelectrophoresis
Cross-reactivityThis antibody cross-reacted with 5 strains of Candida albicans and also cross-reacted with Candida tropicalis and Candida stellatoidia and S. cerevisae but did not cross-react with T. glabrata
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to isolate surface components and determine the chemical structures that are responsible for the immunological differences. This Mab would facilitate the development of serological assays
Curator IDAA + AS
Date of Curation14-06-2010
ReferencesPMC259333


Entry No. - 2   [TOP]
PolysacDB ID1343
Carbohydrate NameStreptococcal cell wall carbohydrate   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeStreptococcus gordonii NCTC 7869   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesAlkali-extracted and purified cell wall polysaccharide from S. gordonii NCTC 7869
Carrier NameNil
Conjugation MethodNil
AntibodiesPolyclonal Antisera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antibody cross-reacted with S. gordonii DL1 and S. oralis ATCC 55229, 34, and ATCC 10557 but did not cross-react with S. mutans NCTC 10449
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antisera was used to study the interactions between Streptococcus and Candida albicans species
Curator IDAA + AS
Date of Curation23-06-2010
ReferencesPMC173231


Entry No. - 3   [TOP]
PolysacDB ID1345
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCandida albicans NIH A-207   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesPolyclonal Antisera
Antibody type and classN/A
Assay SystemOuchterlony double immunodiffusion
Cross-reactivityThe antisera did not cross-react with C. albicans NIH B-792 but cross-reracted with Hormodendrum strains, H. pedrosoi IFO 6071, H. compactum IFO 6726, and H. dermatitidis IFO 6421
Proposed epitopesThe epitope was proposed to consist of α-D-mannopyranosyl residues occupying the nonreducing terminals of the branching moieties
IEDB Epitope76848
Proposed UtilityThis antisera was used to study the structural similarities between Candida mannans and Hormemendrum galactomannans
Curator IDAA + AS
Date of Curation24-06-2010
ReferencesPMC260140


Entry No. - 4   [TOP]
PolysacDB ID1346
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCandida albicans NIH B-792   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB Structure104624
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesPolyclonal Antisera
Antibody type and classN/A
Assay SystemOuchterlony double immunodiffusion
Cross-reactivityThe antisera cross-reacted with C. albicans NIH A-207 and Hormodendrum strains, H. pedrosoi IFO 6071, H. compactum IFO 6726, and H. dermatitidis IFO 6421
Proposed epitopesThe epitope was proposed to consist of α-D-mannopyranosyl residues occupying the nonreducing terminals of the branching moieties
IEDB Epitope76848
Proposed UtilityThis antisera was used to study the structural similarities between Candida mannans and Hormemendrum galactomannans
Curator IDAA + AS
Date of Curation24-06-2010
ReferencesPMC550701


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