| PolysacDB ID | 1091 |
| Carbohydrate Name | Inner core Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Escherichia coli F653 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It consists of the uncommon sugars L-glycero-D mannoheptose (Hep) and 3-deoxy-D-manno-octulosonic acid (Kdo) along with Lipid A
|
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Heat killed bacteria |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab WN1 222-5 |
| Antibody type and class | IgG2a |
| Assay System | ELISA, immunoblotting, passive hemolysis assay |
| Cross-reactivity | WN1 222-5 binds to all E. coli clinical isolates tested so far (79 blood isolates, 80 urinary isolates, and 21 fecal isolates), Salmonella LPS, Shigella LPS and to some Citrobacter, Enterobacter, and KiebsieUla isolates |
| Proposed epitopes | The epitope consists of the inner core |
| IEDB Epitope | 76794 |
| Proposed Utility | In vitro, this Mab inhibits the Limulus amoebocyte lysate assay activity of S-form and R-form LPS in a dose-dependent manner and it neutralizes the LPS-induced release of clinically relevant monokines (interleukin 6 and tumor necrosis factor). In vivo, WN1 222-5 blocks endotoxin-induced pyrogenicity in rabbits and lethality in galactosamine-sensitized mice |
| Curator ID | AA + AS |
| Date of Curation | 27-01-2010
|
| References | PMC281087
|