PolysacDB ID | 2055 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Pseudomonas syringae IMV 7836 pv. Atrofaciens (NCBI Taxonomy) (Drugpedia) |
Basic Structure | O-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
|
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab Ps1a |
Antibody type and class | IgG3 |
Assay System | Agglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting |
Cross-reactivity | "All P. syringae strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi as well as 72 (87.8%), 3 (75%), and 31 (50%) strains with pathovars atrofaciens, striafaciens, and syringae, respectively, and 20 (80%) P. syringae strains for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1a" |
Proposed epitopes | The epitope of Mab 1a is related to the Rha(α1-->2)D-Rha(α1-->3)D-Rha sequence of the O-polysaccharide backbone with the immunodominant site (α1-->2)D-rhamnose(α1-->3) |
IEDB Epitope | N/A |
Proposed Utility | This Mab was useful for elucidation of the LPs structure |
Curator ID | AA + AS |
Date of Curation | 31-12-2010
|
References | PMC178531 |