PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2055
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas syringae IMV 7836 pv. Atrofaciens   (NCBI Taxonomy)   (Drugpedia)
Basic StructureO-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Ps1a
Antibody type and classIgG3
Assay SystemAgglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting
Cross-reactivity"All P. syringae strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi as well as 72 (87.8%), 3 (75%), and 31 (50%) strains with pathovars atrofaciens, striafaciens, and syringae, respectively, and 20 (80%) P. syringae strains for which the pathovar(s) has not been identified cross-reacted strongly with the MAb Ps1a"
Proposed epitopesThe epitope of Mab 1a is related to the Rha(α1-->2)D-Rha(α1-->3)D-Rha sequence of the O-polysaccharide backbone with the immunodominant site (α1-->2)D-rhamnose(α1-->3)
IEDB EpitopeN/A
Proposed UtilityThis Mab was useful for elucidation of the LPs structure
Curator IDAA + AS
Date of Curation31-12-2010
ReferencesPMC178531


Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020