PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2065
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas syringae serogroup O1 strain atrofaciens NCPPB 2612   (NCBI Taxonomy)   (Drugpedia)
Basic StructureO-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Ps1C1
Antibody type and classIgG3
Assay SystemELISA, agglutination and immunoblotting
Cross-reactivityThis Mab showed strong reactivity with OPS of chemotype 1C and weak reactivity with OPS of chemotype 1C-1A with a low content of 1A linear O repeats
Proposed epitopesThe epitope is related to the lateral D-GlcNAc residue and also one or more D-Rha residues of the backbone 1A and is more influenced by the protein-dependent conformation of the OPS chain
IEDB EpitopeN/A
Proposed UtilityThis Mab agglutinated heat-killed homologous bacterial cells
Curator IDAA + AS
Date of Curation04-01-2011
ReferencesPMC94168


Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020