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| PolysacDB ID | 2269 |
| Carbohydrate Name | Y O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Heat-killed S. flexnerf bacteria serotype Y |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-5 |
| Antibody type and class | N/A |
| Assay System | ELISA, FARR assay |
| Cross-reactivity | The rat Mab (MASF Y-5) bound LPS of serotypes 2a, 3b, 4a, and Y, all carrying the group antigen 3,4. It bound to the tetrasaccharides-( abcd), (bcda), [cdab'), (dabc), the octasaccharide-(cdabcdab'), and the decasaccharide-BSA (abcdabcdab ') glycoconjugates |
| Proposed epitopes | This Mab bound to intrachain determinants |
| IEDB Epitope | 76764 |
| Proposed Utility | This Mab would help in the delineation of the antigenic stucture of Y O polysaccharide |
| Curator ID | AA + AS |
| Date of Curation | 15-03-2011 |
| References | 2438343 |
| PolysacDB ID | 2285 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype Y (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3226 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-5 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | MASF Y-5 bound to LPSs of serotypes 2a, 3b, 4a, and Y |
| Proposed epitopes | N/A |
| IEDB Epitope | 76891 |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 18-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2328 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3707 |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Heat killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF Y-5 |
| Antibody type and class | IgM |
| Assay System | ELISA, slide agglutination |
| Cross-reactivity | This Mab cross-reacted with serotype 1a, 2a, 3b, 4a and 5a and Y |
| Proposed epitopes | N/A |
| IEDB Epitope | 76815 |
| Proposed Utility | This Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri |
| Curator ID | AA + AS |
| Date of Curation | 03-04-2011 |
| References | PMC267520 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |