PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID2280
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri serotype Y   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3226
Proposed functionsLPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF B
Antibody type and classIgM
Assay SystemELISA
Cross-reactivityThe MASF B antibody reacted with all S. flexneri LPSs tested except 4bR, the rough mutant
Proposed epitopesα-L-Rhap-1-->2-α-L-Rha residues seemed to be the part of the epitope
IEDB Epitope76891
Proposed UtilityThis Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria
Curator IDAA + AS
Date of Curation17-03-2011
References2437036


Entry No. - 2   [TOP]
PolysacDB ID2326
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeShigella flexneri   (NCBI Taxonomy)   (Drugpedia)
Basic StructureChemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure3707
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab MASF B
Antibody type and classIgM
Assay SystemELISA, slide agglutination
Cross-reactivityThis reacted with all 227 strains tested
Proposed epitopesN/A
IEDB Epitope76815
Proposed UtilityThis Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri
Curator IDAA + AS
Date of Curation03-04-2011
ReferencesPMC267520


Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020