| PolysacDB ID | 2309 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype 2a strain 2457T (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
|
| BCSDB Structure | N/A |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab HYSF2a2 |
| Antibody type and class | IgM |
| Assay System | ELISA and immunoblotting |
| Cross-reactivity | HySF2a2 showed the most restricted specificity, reacting only with S. flexneri 2a LPS |
| Proposed epitopes | This Mab is likely recognizing an epitope influenced by the presence of a single α-D-glycosyl residue on one of the rhamnose residues |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may have implications for designing a Shigella vaccine that will be protective against related serotypes. This Mab may also be used to study the ultrastructural surface distribution of LPSs on the surface of the bacteria |
| Curator ID | AA + AS |
| Date of Curation | 29-03-2011
|
| References | PMC170411 |