| PolysacDB ID | 2076 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Pseudomonas aeruginosa (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
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| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Healthy adult human individual B cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab FKF-lF3 |
| Antibody type and class | IgM |
| Assay System | ELISA, immunoblotting, animal protection studies |
| Cross-reactivity | This Mab cross-reacted with N-acetyl-β-glucosamine- conjugated bovine serum albumin, N-acetyl-β-galactosamine-conjugated bovine serum albumin, myosin and actin, but not with other autoantigens such as ss- and ds-DNA, cardiolipin and glycosaminoglycans. This Mab bound to many serotype strains of P. aeruginosa namely, A, F, G and M serotypes, in addition to E. coli Rc type rough mutant, strain J5" |
| Proposed epitopes | This Mab recognised the outer core regions of some serotype strains and the O-polysaccharide region of serotype A strains. A carbohydrate structure containing N-acetylhexosamine residues, such as GlcNAc and GalNAc, was the most likely epitope for FKF-lF3 |
| IEDB Epitope | N/A |
| Proposed Utility | The mAb conferred protective activity against a mouse pseudomonal infection model. The mAb was a naturally occurring polyspecific antibody that participated in defense against pseudomonal infections |
| Curator ID | AA + AS |
| Date of Curation | 10-01-2011
|
| References | 8804973 |