|
|
|
|
Home | Search | Advance Search | Browse | Online Submission | Documentation & FAQ | Team | Contact us |
|
| PolysacDB ID | 1013 |
| Carbohydrate Name | Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The GXM backbone consists of a linear α(1-->3)-linked mannan substituted at 2-O positions by single residues of either xylose or glucuronic acid |
| BCSDB Structure | 116826 |
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Pure Capsular polysaccharide |
| Carrier Name | N/A |
| Conjugation Method | N/A |
| Antibodies | Mab E1 |
| Antibody type and class | IgG1 |
| Assay System | Agglutination with C. neoformans serotype A cells, Radial immunodiffusion, Indirect Immunofluorescence,, ELISA, Competitive binding assays |
| Cross-reactivity | This antibody was highly specific to serotype A, low levels of cross reactivity to serotypes B and D. Among the other yeasts tested, a cross-reaction was only detected with Trichosporon beigelii. |
| Proposed epitopes | N\A |
| IEDB Epitope | 115576 |
| Proposed Utility | Could be useful for fundamental studies on the glucuronoxylomannan structure, as well as for clinical applications such as serotyping and possibly the serological diagnosis of cryptococcosis |
| Curator ID | AA + AS |
| Date of Curation | 04-01-2010 |
| References | PMC260404 |
| PolysacDB ID | 2210 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Salmonella minnesota Re chemotype 595 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | LPS is a complex macromolecule composed of the biologically active lipid A moiety, covalently attached core oligosaccharide, and distally linked polysaccharide O-side chain. The core oligosaccharide part consists of the following residues : α-Kdop-(2-8)-α-Kdop-(2-->4)-Kdo |
| BCSDB Structure | N/A |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab E1 |
| Antibody type and class | IgG3 |
| Assay System | Direct and inhibition enzyme immunoassays |
| Cross-reactivity | MAb E1 reacted exclusively with those of Re-chemotype |
| Proposed epitopes | It recognized α-Kdo- monosaccharide and disaccharide structures present as non-reducing side chains in various Re-type LPSs and synthetic antigens |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be used for identification of Salmonella strains having the Re chemotype |
| Curator ID | AA + AS |
| Date of Curation | 04-03-2011 |
| References | 1294455 |
|
Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |