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| PolysacDB ID | 2508 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Vibrio cholerae 01 serogroup (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues. |
| BCSDB Structure | 6642 |
| Proposed functions | V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera |
| Antigenic Nature used to produce antibodies | Killed Vaccine consisting of V. cholerae O1 Inaba (ATCC 14033) and Ogawa (ATCC 14035) cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab A6 |
| Antibody type and class | IgG3 |
| Assay System | Enzyme-linked immunosorbent assay (ELISA), immunofluorescence, Immunoelectron microscopy and the slide agglutination test |
| Cross-reactivity | This Mab reacted with both Inaba and Ogawa serovars (A antigen) and was specific to O1 serotype |
| Proposed epitopes | The epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab produced discernible agglutination within 1 min with each of the 37 01 whole-cell suspensions tested |
| Curator ID | AA + AS |
| Date of Curation | 10-06-2011 |
| References | PMC266720 |
| PolysacDB ID | 2560 |
| Carbohydrate Name | Exopolysaccharide xanthan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Xanthomonas campestris pv. campestris 646 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | It consists of a β1-->4 glucose polymer, i.e. a cellulose backbone, with β1-->3 substituted trisaccharide side-chains of (mannose-β1-->4 glucuronic acid-α-1-->2-mannose-β-1-->3)- on alternate glucose residues. The side-chain is variably acylated. The inner mannose may have an O-6 acetyl group and the terminal mannose a 4,6- bound pyruvate ketal |
| BCSDB Structure | N/A |
| Proposed functions | The exopolysaccharide xanthan from Xanthomonas campestris is an interesting carbohydrate polymer, with striking rheological properties which make it useful as a food additive (gelling agent) and also as a potential vehicle in enhanced oil recovery |
| Antigenic Nature used to produce antibodies | Xanthan |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab A6 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | This Mab was specific to Xanthan and did not cross-react with Cellulose |
| Proposed epitopes | The specificity was mainly against the side-chain of xanthan. This Mab was assumed to be directed against the nonsubstituted trisaccharide with the inner mannose-glucuronic acid being immunodominant |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab could be useful for structural studies, for detection purposes in commercial and agricultural settings, and for serological typing purposes |
| Curator ID | AA + AS |
| Date of Curation | 28-06-2011 |
| References | 2621443 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |