PolysacDB ID | 2209 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Salmonella enteritidis (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Salmonella enteritidis lipopolysaccharide is composed of high-molecular-weight (HMW) LPS and low-molecular-weight (LMW) LPS. The HMW LPS O-antigen polysaccharides are comprised of two different repeating units, -[-->2)-[α-Tyvp-(1-->3)]beta-D-Manp-(1-->4)-α-L-R hap-(1-->3)-α-D-Galp-(1-->]- (structure I) and [-->2)-[α-Tyvp-(1-->3)]β-D-Manp-(1-->4)-α--L-R hap-(1-->3)-[α-D-Glcp-(1-->4)]α-D-Galp-(1-->]- (structure II). The LMW LPS contains truncated O-antigen polysaccharide which is comprised of only structure I. |
BCSDB Structure | 128190 |
Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
Antigenic Nature used to produce antibodies | Live attenuated mutant strain of Salmonella typhimurium |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 8aC10 |
Antibody type and class | N/A |
Assay System | ELISA, immunoblotting, passive hemagglutination (PHA), passive hemolysis and agglutination tests |
Cross-reactivity | The MAb reacted only with lipopolysaccharides (LPS) of Salmonella strains from group O:4 (B), expressing O:5 antigen. The MAb agglutinated in addition Salmonella strains with O:8 antigen from group C(2)-C(3) but did not react with purified LPS |
Proposed epitopes | This Mab is directed to Salmonella O:5 antigen of the LPS. The epitope is possibly localized onto O-acetyl-abequose and abequose residues bound with a α-1-->3 linkage to the basic polysaccharide backbone of Salmonella LPS with O:5 and O:8 antigen respectively |
IEDB Epitope | N/A |
Proposed Utility | This Mab can be used for identification of Salmonella strains having O:5 LPS |
Curator ID | AA + AS |
Date of Curation | 04-03-2011
|
References | 12951256 |