PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1527
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeKlebsiella pneumoniae O1:K2   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsIt has been shown to be an important virulence factor and potent antigen for the induction of immunoprotection
Antigenic Nature used to produce antibodiesLPS-associated proteins plus LPS
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 3C2
Antibody type and classIgM
Assay SystemELISA
Cross-reactivityThis Mab cross-reacted with K. pneumoniae R:K18, a rough strain. This Mab did not cross-react with LPS from Klebsiella strains of the 02, 03, and 04 serotypes nor LPS from E. coli 0111:B4
Proposed epitopesThis Mab recognized the LPS core
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation23-08-2010
ReferencesPMC313574


Entry No. - 2   [TOP]
PolysacDB ID2604
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCryptococcus neoformans Serotype C   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe capsular polysaccharide has a linear α-(1-->3)-linked mannose backbone that is substituted with nonreducing D-xylosyl and D-glucuronosyl acid groups. The mannose backbone is partially O-acetylated
BCSDB Structure136942
Proposed functionsThe capsular polysaccharide is an important virulence factor
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameSheep erythrocytes
Conjugation MethodThe capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature
AntibodiesMab 3C2
Antibody type and classN/A
Assay SystemELISA and Immunodiffusion
Cross-reactivityThis antibody cross-reacted with Serotype A,B,C and D serotypes
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityUsed to differentiate various strains of all serotypes. This monoclonal antibody promoted opsonization of Cryptococcus cells
Curator IDAA + AS
Date of Curation16-07-2011
References2476401


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