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| PolysacDB ID | 1119 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Actinobacillus pleuropneumoniae serotype 1 shope 4074 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The O-polsaccharide structure is as follows : --6)-α-D-Glcp-(1-->2)-α-L-Rhap-(l-->2)-α-L-Rhap-(1--. which is linked to 1-D-GlcpNAc chains on its rhamnopyranosyl residue via 1-->3 linkage. |
| BCSDB Structure | N/A |
| Proposed functions | LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus |
| Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 1 cells (strain Shope 4074] |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 3B10 |
| Antibody type and class | IgM |
| Assay System | ELISA, Rapid Dot ELISA and Slide agglutination test |
| Cross-reactivity | This antibody cross-reacted with reference strains of serotypes 1, 9 and 11 |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may provide reliable specific tools for rapid serological diagnosis of animals infected with serotypes 1, 9 and 11 by inhibition or competition ELISA. Moreover, these mAbs could be useful as a complementary specific reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotypes 1, 9 and 11 |
| Curator ID | AA + AS |
| Date of Curation | 04-02-2010 |
| References | 9116634 |
| PolysacDB ID | 1498 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype A [strain 24064] (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues |
| BCSDB Structure | N/A |
| Proposed functions | C. neoformans has a large polysaccharide capsule that is a determinant of virulence. The capsular polysaccharide causes immunologic paralysis and inhibits phagocytosis by macrophages. Soluble C. neoformans capsular polysaccharide enhances human immunodeficiency virus [HIV] infection in cultured cells, suggesting a new pathogenic role in AIDS |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 3B10 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | This antibody reacted with 2 serotype A strains 24064 and 371 |
| Proposed epitopes | This Mab did not bind to de-O-acetylated GXM from both serotype A strains. This shows that acetylated residues form an important part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | N/A |
| Curator ID | AA + AS |
| Date of Curation | 04-08-2010 |
| References | 1583327 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |